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ROTI®Lux SYBR®Green qPCR Master Mix, 1 ml, 1 x 1 ml

2x conc., ready to use, no ROX
Icon_Kuehltransport The products marked accordingly are shipped refrigerated in special, sustainable cooling boxes with cooling packs or dry ice.
Icon_ready-to-use Ready-to-use products and reagents
Pack Qty.
Pack.
Packaging
Density (D) ~1 g/cm³
Boiling point (bp) 100 °C
Storage temp. -20 °C
Transport temp. cooled
WGK 1

For ultra-fast qPCR protocols with excellent sensitivity and melting curve analyses (HRM) for genotyping.
The ROTI®Lux qPCR Master Mixes are stable for several weeks at 4 °C. For short-term storage, we recommend storing at 2 - 8 °C in the refrigerator.

ROTI®Lux SYBR®Green qPCR Master Mixes are suitable for all common types of quantitative gene expression analyses and genotyping using real-time PCR.
The qPCR Master Mixes contain an optimised DNA polymerase that enables ultra-fast runs in less than 20 minutes. Due to the particularly high sensitivity, just one template per reaction is sufficient for the analysis.
The Master Mixes are also characterised by high temperature stability, which enables pipetting at room temperature and means that they remain stable even after several reeze and Thawcycles (>10x).
Product details

€80.65/Pack Qty. 

excl. VAT. | 1 ml per Pack Qty.

Art. No. 25KX.1

In production
Additional transportation costs apply for this item.
from 6 Pack Qty. €76.62/Pack Qty.
from 24 Pack Qty. €72.58/Pack Qty.

Product details


ROTI®Lux SYBR®Green qPCR Master Mixes are suitable for all common types of quantitative gene expression analyses and genotyping using real-time PCR.
The qPCR Master Mixes contain an optimised DNA polymerase that enables ultra-fast runs in less than 20 minutes. Due to the particularly high sensitivity, just one template per reaction is sufficient for the analysis.
The Master Mixes are also characterised by high temperature stability, which enables pipetting at room temperature and means that they remain stable even after several reeze and Thawcycles (>10x).

Properties:
  • High temperature stability
  • Excellent sensitivity (<0.1 pg cDNA)
  • Ultra-fast qPCR in <20 min
  • Suitable for melting curve analysis (HRM)
  • For all common qPCR cyclers - optionally with or without ROX

Applications:
  • Gene expression analyses
  • Melting curve analyses (HRM)
  • Genotyping
  • Concentration determination of NGS libraries
  • Determination of copy number variants
  • Telomere length measurement
  • Pathogen detection
  • Quality control


ROTI®Lux SYBR®Green qPCR Master Mix 2x conc., ready to use, no ROX

Master Mix without the reference dye ROX (6-Carboxy-X-rhodamine)

Suitable for:
  • Exicycler™(Bioneer)
  • CFX Real-Time PCR Cycler (Biorad)
  • RealLine Cycler (Bioron)
  • Dtlite & Dtprime Cycler (DNA Technology)
  • Mastercycler® (Eppendorf)
  • LightCycler® (Roche)
  • AriaMx Real-time PCR System (Agilent)
  • Q-Cycler (Quantabio)
25KP_02
Stability by multiple freeze and thaw cycles: The stability of the ROTI®Lux qPCR master mixes is guaranteed even after multiple freeze and thaw cycles (≥10).

25KX_03
Sensitivity and dynamic range: The ROTI®Lux SYBR®Green qPCR Master Mixes deliver consistent and reliable results, even at low input amounts such as 0.1 pg cDNA, or 1 template per reaction.

25KP_01
Stability at room temperature: The ROTI®Lux qPCR Mastermixes can be stored at room temperature (≤30 °C) for at least 14 days.


Not a medical device / Not an IVD product
Technical Information
Application Gene expression analyses, SNP analyses, telomere length measurement 
Basic material DNA (cDNA / gDNA) 
Polymerase HotStart DNA Polymerase 
Instructions for use, addition The master mix contains a modified HotStart DNA polymerase, dNTPs, MgCl2 and the reaction buffer with the SYBR®Green dye. Primer and template must be added separately.
1 ml Master Mix is sufficient for one 96-well plate. 
ROTI®Lux SYBR®Green qPCR Master Mix
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Art. No. Pack Qty. Pack. Packaging Price Quantity
25KX.1 1 ml plastic 1 x 1 ml

€80.65

25KX.2 5 ml plastic 5 x 1 ml

€376.25

25KX.3 10 ml plastic 10 x 1 ml

€698.75

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Selected quantity:   0
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