Technical Data Sheet
ROTI®Lux SYBR®Green qPCR Master Mix, 1 ml, 1 x 1 ml
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Ready-to-use products and reagents
Boiling point (bp) 100 °C
Storage temp. -20 °C
Transport temp. cooled
WGK 1
For ultra-fast qPCR protocols with excellent sensitivity and melting curve analyses (HRM) for genotyping.
The qPCR Master Mixes contain an optimised DNA polymerase that enables ultra-fast runs in less than 20 minutes. Due to the particularly high sensitivity, just one template per reaction is sufficient for the analysis.
The Master Mixes are also characterised by high temperature stability, which enables pipetting at room temperature and means that they remain stable even after several reeze and Thawcycles (>10x).
€60.45/Pack Qty. Campaign price!
excl. VAT. | 1 ml per Pack Qty.
Art. No. 25KY.1
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Product details
ROTI®Lux SYBR®Green qPCR Master Mixes are suitable for all common types of quantitative gene expression analyses and genotyping using real-time PCR.
The qPCR Master Mixes contain an optimised DNA polymerase that enables ultra-fast runs in less than 20 minutes. Due to the particularly high sensitivity, just one template per reaction is sufficient for the analysis.
The Master Mixes are also characterised by high temperature stability, which enables pipetting at room temperature and means that they remain stable even after several reeze and Thawcycles (>10x).
- High temperature stability
- Excellent sensitivity (<0.1 pg cDNA)
- Ultra-fast qPCR in <20 min
- Suitable for melting curve analysis (HRM)
- For all common qPCR cyclers - optionally with or without ROX
- Gene expression analyses
- Melting curve analyses (HRM)
- Genotyping
- Concentration determination of NGS libraries
- Determination of copy number variants
- Telomere length measurement
- Pathogen detection
- Quality control
ROTI®Lux SYBR®Green qPCR Master Mix 2x conc., ready to use, low ROX
Master mix with low concentration (100 nM) of the reference dye ROX (6-Carboxy-X-rhodamine)
- Quantstudio™ Cycler ( Thermofisher)
- qTower (Analytik Jena)
- Mx3000P® qPCR Systeme (Agilent)
- Rotor-Gene™ (Qiagen)
| Application | Gene expression analyses, SNP analyses, telomere length measurement |
| Basic material | DNA (cDNA / gDNA) |
| Polymerase | HotStart DNA Polymerase |
| Instructions for use, addition |
The master mix contains a modified HotStart DNA polymerase, dNTPs, MgCl2 and the reaction buffer with the SYBR®Green dye. Primer and template must be added separately. 1 ml Master Mix is sufficient for one 96-well plate. |
| Version | low ROX |
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| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 25KY.1 | 1 ml | plastic | 1 x 1 ml |
€80.65
€60.45 |
|
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| 25KY.2 | 5 ml | plastic | 5 x 1 ml |
€376.25
€282.15 |
|
|
| 25KY.3 | 10 ml | plastic | 10 x 1 ml |
€698.75
€524.05 |
|
|
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