Jump to content Jump to navigation menu

ROTI®Free Stripping Buffer 2.0, 250 ml

ready-to-use
Icon_ready-to-use Ready-to-use products and reagents
Examples of effect: Damage metals and burn body tissues; may cause serious eye damage. Safety: Avoid contact; wear safety spectacles and gloves. In the event of contact with eyes and skin, rinse with water.
Warning
H290-H319
i may be corrosive to metals, causes serious eye irritation
P305+P351+P338 P337+P313 P406
i IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, if eye irritation persists: Get medical advice/attention, store in a corrosion-resistant container with a resistant inner liner
Pack Qty.
Pack.
Stripping Buffer
Density (D) 1 g/cm³
Boiling point (bp) 100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
ADR 8 III
WGK 1
UN-Nr. 1760

Premixed buffer for gentle stripping of Western-Blot membranes after Chemoluminescence detection.
Suitable for nitrocellulose and PVDF-membranes.

Stripping buffer for efficient removal of primary and secondary antibodies, as well as chemoluminescence staining, from Western blot membranes. Due to the low pH of the buffer, the antibody conformation is altered, therefore gently removing all molecules bound to the epitopes (Legocki et. al. (1981) Analyt. Biochem. 111:385-92). Robust formulation without β-mercaptoethanol or DTT for use at room temperature. Suitable for nitrocellulose and PVDF membranes.
Product details

€47.85/Pack Qty. 

excl. VAT. | 250 ml per Pack Qty.

Art. No. 3319.1

In stock
Delivery fast, simple and reliable!
from 6 Pack Qty. €45.46/Pack Qty.
from 24 Pack Qty. €43.06/Pack Qty.
Carl Roth subscription service

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:
1

Put all products for your subscription in the desired quantity in the basket.

2

In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.

3

Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Product details



ROTI®Free Stripping Buffer 2.0 ready-to-use

Stripping buffer for efficient removal of primary and secondary antibodies, as well as chemoluminescence staining, from Western blot membranes. Due to the low pH of the buffer, the antibody conformation is altered, therefore gently removing all molecules bound to the epitopes (Legocki et. al. (1981) Analyt. Biochem. 111:385-92). Robust formulation without β-mercaptoethanol or DTT for use at room temperature. Suitable for nitrocellulose and PVDF membranes.

  • Effective
  • Gentle
  • Odour-free
  • Simple application w/o heating
  • Cost-efficient

Directions for use

Following rinsing steps in PBST- or TBST buffer, the membrane is incubated in ROTI®Free Stripping-Puffer 2.0 for 15-60 mins. at room temperature. After washing it again, the membrane may be blocked and probed anew. The particular time needed for complete stripping depends on the respective antibody/epitope pairing.



ROTI®Free Stripping Buffer 2.0
Selected quantity:   0
  1. Subtotal:  0.00
Order No. Pack Qty. Pack. Price Quantity
3319.1 250 ml glass

€47.85

3319.2 1 l glass

€124.20

In stock
In procurement
Not available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Notes on the product

Remark: Contains SDS. Can be redissolved by heating (max. 45 °C).