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ROTI®Free Stripping Buffer 2.2 plus, 250 ml

ready-to-use
Icon_ready-to-use Ready-to-use products and reagents
Pack Qty.
Pack.
Stripping Buffer
Density (D) 1,012 g/cm³
Boiling point (bp) 100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 1

Premixed buffer for efficient stripping of Western-Blot membranes after Chemoluminescence detection, even with hard-to-remove antibodies.
Suitable for nitrocellulose and PVDF-membranes.

Stripping buffer for removal of high-affinity antibodies, as well as chemoluminescence staining, from Western blot membranes. Due to the low pH of the buffer, the antibody conformation is altered, therefore gently removing all molecules bound to the epitopes (Legocki et. al. (1981) Analyt. Biochem. 111:385-92). Specially designed additives enhance the efficiency in removing those antibodies requiring long stripping times or high incubation temperatures with standard stripping buffer systems. Advanced formulation without β-mercaptoethanol or DTT for use at room temperature or heated. Suitable for nitrocellulose and PVDF membranes.
Product details
Notes on the product

€52.60/Pack Qty. 

excl. VAT. | 250 ml per Pack Qty.

Art. No. 3337.1

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Product details



ROTI®Free Stripping Buffer 2.2 plus ready-to-use

Stripping buffer for removal of high-affinity antibodies, as well as chemoluminescence staining, from Western blot membranes. Due to the low pH of the buffer, the antibody conformation is altered, therefore gently removing all molecules bound to the epitopes (Legocki et. al. (1981) Analyt. Biochem. 111:385-92). Specially designed additives enhance the efficiency in removing those antibodies requiring long stripping times or high incubation temperatures with standard stripping buffer systems. Advanced formulation without β-mercaptoethanol or DTT for use at room temperature or heated. Suitable for nitrocellulose and PVDF membranes.

  • Very effective
  • Gentle
  • Odour-free
  • Simple application
  • Also suitable for high-affinity antibodies

Directions for use

Following rinsing steps in PBST- or TBST buffer, the membrane is incubated in ROTI®Free Stripping-Puffer 2.2 plus for 30-60 mins. at room temperature. In case of very persistent antibodies incubation in heated buffer is allowed. After washing it again, the membrane may be blocked and probed anew. The particular time needed for complete stripping depends on the respective antibody/epitope pairing.



ROTI®Free Stripping Buffer 2.2 plus
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3337.1 250 ml glass €52.60
3337.2 1 l glass €130.65
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Notes on the product

Remark: Contains SDS. Can be redissolved by heating (max. 45 °C).

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