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ROTI®Free Stripping Buffer, 250 ml

ready-to-use
Icon_ready-to-use Ready-to-use products and reagents
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H317-H319-H412
i may cause an allergic skin reaction, causes serious eye irritation, harmful to aquatic life with long lasting effects
P280 P302+P352 P305+P351+P338
i wear protective gloves/eye protection, IF ON SKIN: Wash with plenty of water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Stripping Buffer
Density (D) 1 g/cm³
Boiling point (bp) 100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 2

Premixed buffer for stripping of Western-Blot membranes.
Suitable for nitrocellulose and PVDF-membranes.

The buffer offers a simple tool for removing antibodies and chemoluminescence staining from Western blot membranes, allowing the valuable membranes to be analysed several times. Excellent when several proteins are to be detected in the same extract, or e.g. when in addition to a specific analysis, proteins in the respective lanes have to be semi-quantified (Kaufmann et. al. (1987) Anal. Biochem. 161:89-95). Suitable for nitrocellulose and PVDF-membranes.
Product details

€21.00/Pack Qty. 

excl. VAT. | 250 ml per Pack Qty.

Art. No. 0083.2

Available at short notice
from 6 Pack Qty. €19.95/Pack Qty.
from 24 Pack Qty. €18.90/Pack Qty.
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Product details



ROTI®Free Stripping Buffer ready-to-use

The buffer offers a simple tool for removing antibodies and chemoluminescence staining from Western blot membranes, allowing the valuable membranes to be analysed several times. Excellent when several proteins are to be detected in the same extract, or e.g. when in addition to a specific analysis, proteins in the respective lanes have to be semi-quantified (Kaufmann et. al. (1987) Anal. Biochem. 161:89-95). Suitable for nitrocellulose and PVDF-membranes.

  • Efficient
  • Well-tried formulation
  • With β-mercaptoethanol
  • For stripping under heating
  • Low-priced

Directions for use

Wash the membrane in TBST-buffer (Art. No. 1061.1), incubate in ROTI®Free Stripping-buffer in closed hood for 30 min at 56 °C (e. g. in shaking water bath). To strip AP-conjugates, we recommend incubation at 70 °C for 30-60 min. After washing the membrane twice in TBST for 20 min each time, it should first be blocked, before the first antibody is added. The stripping process can be checked by brief chemiluminescence staining after washing in TBST.
A slight reduction of the membrane-bound proteins and increase of the background may appear after repeated stripping. Store membranes after stripping wetpacked at +2 to +8 °C.



ROTI®Free Stripping Buffer
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Order No. Pack Qty. Pack. Price Quantity
0083.1 1 l glass €65.05
0083.2 250 ml glass €21.00
0083.3 2.5 l glass €114.50
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Notes on the product

Remark: Contains SDS. Can be redissolved by heating (max. 60 °C).

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