Primin

For reference substances

HPLC-chromatogram, TLC-chromatogram and GC-chromatogram respectively

Reference substances

ROTICHROM^® HPLC/TLC/GC

Pure reference substances are essential for precise analysis. They are used for identifying compounds by retention time, for calibrating detectors, and for eliminating errors in quantification of the sample compounds.

Peak areas are proportional to sample quantity in all chromatographic methods. For precise quantitative analysis, however, it is necessary to calibrate the detector as the signal may significantly vary with different substances.

To avoid errors during sample preparation and injection, an internal standard should be used in any case. The standard must not be present in the sample, however, its chemical and physical properties ought to be the closest possible to the analytical targets. It must be well resolved in the chromatogram within the same range of retention time as the sample. Before sample analysis, a given volume of standard is added, and all clean-up procedures are made with the sample containing the standard. Quantitative errors may be compensated by comparing the peak-areas of the sample with that of the internal standard.

Reference Substances with Analytical Documentation

TLC, HPLC, ¹H-NMR, ¹³C-NMR, UV, IR, MS
The high-purity substances in this product range are supplied with detailed analytical documentation. The documentation comprises approx. 20 pages and contains all important data on the analytical methods used. The results are usually confirmed by at least oneadditional method. This ensures the greatest possible certainty in determining the content and checking the identity.