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ROTI®Cell Water, 500 ml

CELLPURE® ready-to-use, sterile
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Empirical formula H2O
Molar mass (M) 18,02 g/mol
Density (D) 1 g/cm³
Boiling point (bp) 100 °C
Melting point (mp) 0 °C
Storage temp. +2 to +25 °C
Transport temp. ambient temp.
CAS No. 7732-18-5
EG-Nr. 231-791-2

Water for cell culture
Endotoxins: <0.25 E.U./ml

€17.75/Pack Qty. 

excl. VAT. | 500 ml per Pack Qty.

Art. No. 9186.1

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Product details

Sterile


ROTI®Cell Water CELLPURE® ready-to-use, sterile

Not a medical device / Not an IVD product
Technical Information
Instructions for use, addition Once opened, store at +2 °C to +8 °C and use within 2 years. 
ROTI®Cell Water
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9186.1 500 ml plastic

€17.75

9186.2 1 l plastic

€25.30

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Downloads / MSDS


General information

The buffer system in cell culture media

In addition to the various nutrients in the medium, the pH value also plays a very important role in cell culture. The physiological pH for almost all mammalian cells is 7.4. Deviating from this, it has been observed that certain transformed cell lines prefer a pH between 7.0 and 7.4 and certain fibroblasts prefer a pH between 7.4 and 7.7. In any case, pH fluctuations should be avoided and the pH should be maintained as well as possible. That is why a buffer system in media is necessary. The most important buffer system in the body is the carbonic acid-carbonate buffer system. Accordingly, almost all cell culture media contain bicarbonate (NaHCO3). Bicarbonate alone leads to a strong pH increase of the medium, so that NaHCO3-buffered media must always be used with CO2 incubators. Depending on the NaHCO3 concentration in the medium, different CO2 environments are necessary to ensure a pH of about 7.4. For this reason, you should not work outside of the CO2 incubator for too long. If this is necessary, a HEPES buffer should be used in addition to the NaHCO3, which contributes as a second buffer system to stabilize the pH value.


Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

Sterilitycomplies
Endotoxins<1,0 EU/ml