Jump to content Jump to navigation menu

ROTI®Fair TG-Western

for 5000 ml/pouch, for electrophoresis
Icon_ROTH-for-Future Products with optimised manufacturing process in terms of sustainability.
Pack Qty.
Pack.
TG buffer powder (pouch), Tris-glycine buffer
Density (D) 1,19 g/cm³
WGK 1

Buffer for protein electrophoresis and transfer.
1x TG-Western solution is prepared by dissolution of 1 pouch in 5000 ml highly pure water.

€428.95/Pack Qty. 

excl. VAT. | 10 unit(s) per Pack Qty.

Art. No. 1276.1

In stock
Delivery fast, simple and reliable!
from 6 Pack Qty. €407.50/Pack Qty.
from 24 Pack Qty. €386.06/Pack Qty.
Carl Roth subscription service

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:
1

Put all products for your subscription in the desired quantity in the basket.

2

In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.

3

Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Product details



ROTI®Fair TG-Western for 5000 ml/pouch, for electrophoresis

Technical Information
Batch quantity 5000 ml/pouch 
End concentration 1x 
pH value 8,3 ±0,05 
Buffer/solution Tris-glycine buffer 
Use Buffer for protein electrophoresis and transfer 
ROTI®Fair TG-Western
Selected quantity:   0
  1. Subtotal:  0.00
Order No. Pack Qty. Pack. Price Quantity
1276.1 10 unit(s) box

€428.95

In stock
In procurement
Not available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS


General information


Buffer Recommendations for Transfer (Blotting)

Buffers recommended for Tank blotting:
Acc. to Tobwin et al., 1997: 25 mM Tris, 192 mM glycin, 20 % methanol. Optional: 0-0,1 % SDS.
Acc. to Bjerrum und Schaefer-Nielsen, 1986: 48 mM Tris, 39 mM glycin, 0-20 % methanol. Optional: 0-0.1 % SDS.
Acc. to Dunn, 1986: 10 mM NaHCO3, 3 mM NaCO3, 20 % methanol.

Buffers recommended for Semy Dry blotting:
Discontinuous buffer system: ROTI®Blot 1 - for standard proteins or ROTI®Blot 2 - for hydrophobic proteins.
Continuous Tris-glycin buffer acc. to Bjerrum and Schaefer-Nielsen, 1986: 48 mM Tris, 39 mM glycin, 0-20 % methanol. Optional: 0,01-0,1 % (usually 0,0375 %) SDS.
Continuous CAPS buffer for blotting of basic proteins and prior to N-terminal sequencing: 0,22 % CAPS (pH 10,5-11,0), 10 % methanol.
Do not adjust pH of the buffer (exception: CAPS stock solution). Adding acid or base to the buffer will result in higher conductivity, leading to increased formation of heat as well as ions. Subsequently, this results in brown, “burnt” looking blotting paper and damage to the electrode plates.

Methanol prevents the gel from swelling during transfer (mandatory for gradient gels!) and improves the absorption of proteins to NC membranes. For transfer of native or big proteins, reduce or omit methanol.

SDS improves transfer efficiency of large proteins and protein binding to PVDF membranes, however increasing relative current, power and temperature. Don’t use SDS for Semi-Dry blotting of small proteins to NC membranes. For tank blotting use 0,02 % SDS in minimum.

For blotting of native proteins use buffer acc to Bjerrum and Schaefer-Nielsen with 0,04 % SDS and 0-10 % methanol.


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Composition

Prepared 1x TG-Western solution contains: 25 mM Tris, 0.192 M glycine, pH-value 8.3 ±0.05, when prepared in deionized or distilled water.