Jump to content Jump to navigation menu

ROTI®Fect, 0.5 ml, 1 x 0,5 ml

CELLPURE® ready-to-use, for transfection
Icon_ready-to-use Ready-to-use products and reagents
Pack Qty.
Pack.
Packaging
Transfection reagents
Density (D) 1 g/cm³
Storage temp. +4 °C
WGK 3

Reagent for liposoma-mediated transfection of eukaryotic cells.
Approx. 400 assays per ml in 24wells. Dependent on the cell line used.

ROTI®Fect is a liposome formulation of a polycationic lipid in combination with a neutral colipid. Compared to monocationic lipid formulations, ROTI®Fect has a higher efficiency with lower levels being required. Its excellent reproducibility in combination with its high level of efficiency reduced optimisation work to a minimum. Because of its low toxicity, a wide range of cell lines can be transfected efficiently.
An efficient triple-cotransfection using ROTI®Fect is described in Ufartes, Schlüter, Kaulfuss and Seipp (2005) Biospektrum 4: 464-5.
Product details
€209.65 /Pack Qty.

€167.70/Pack Qty.  Campaign price!

excl. VAT. | 0.5 ml per Pack Qty.

Art. No. P001.2

In stock
Delivery fast, simple and reliable!

Product details



ROTI®Fect CELLPURE® ready-to-use, for transfection

ROTI®Fect is a liposome formulation of a polycationic lipid in combination with a neutral colipid. Compared to monocationic lipid formulations, ROTI®Fect has a higher efficiency with lower levels being required. Its excellent reproducibility in combination with its high level of efficiency reduced optimisation work to a minimum. Because of its low toxicity, a wide range of cell lines can be transfected efficiently.
An efficient triple-cotransfection using ROTI®Fect is described in Ufartes, Schlüter, Kaulfuss and Seipp (2005) Biospektrum 4: 464-5.

  • Highly efficient reproduction of DNA in mammal cells
  • Excellent reproducibility
  • High and wide operating plateau
  • No inhibition through serum
  • Low cell toxicity
  • Successful with different cell lines
  • Excellent transfection results

Successfully tested on a variety of cell types.

siRNA: CEM, COS-7, ES-D3,HeLa, HL60, LNCaP, OVCAR3, PC3, SW620
Primary cells: pAML, pHDF, pMEF, pRHC, pSMC
Cell lines: 143B, 293-T, A-293, A-431, AM-C6SC8, BHK, BV-2, C2C12, C6, CHO, CHO-DHFR, COS-1, COS-7, CRFK, Colo-205, DU-145, ECV-304, EMC, F98 (glioma), GD25-ß1, HBL 100, HCT-116, HCT-15, HEK293, HeLa, HeLa-S3, Hep-3B, Hep-G2, HOSE, HSG, HT-22, HT-29, HUVEC, JURKAT, LLC-PK1, LS174T, LoVo, MCF-7, MDCK, MeWo, MV3, NIH-3T3, NS20Y, OK, P815, PC3, PT-11, Rcho-1, SAOS, SH-SY5Y, SK-MEL-28, SKOV-3, SM10, SW-480, THP-1, tsA201, U87 (glioma), Vero 76, et al.


P001_RotiFect_Ablaufschema
P001_Diagramm
Figure:
Transfection of a variety of cell lines with ROTI®Fect and other commercially available transfectants.
Transfection was performed in 24wells using 0.25 µg DNA each, with 1.5 µl ROTI®Fect or acc. to instructions given by the manufacturers. Luciferase activity was measured 48 hours post transfection. Data of cells transfected with ROTI®Fect was set to 100 %.


ROTI®Fect
Selected quantity:   0
  1. Subtotal:  0.00
Art. No. Pack Qty. Pack. Packaging Price Quantity
P001.1 0.2 ml plastic 1 x 0,2 ml
€97.75

€78.15

P001.2 0.5 ml plastic 1 x 0,5 ml
€209.65

€167.70

P001.3 1 ml plastic 1 x 1 ml
€327.90

€262.30

P001.4 5 ml plastic 5 x 1 ml
€1,451.25

€1,161.00

In stock
Available
In procurement
No longer available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found: