ROTI®Fect, 5 ml, 5 x 1 ml
Approx. 400 assays per ml in 24wells. Dependent on the cell line used.
excl. VAT. | 5 ml per Pack Qty.
Art. No. P001.4
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ROTI®Fect CELLPURE® for transfection
ROTI®Fect is a liposome formulation of a polycationic lipid in combination with a neutral colipid. Compared to monocationic lipid formulations, ROTI®Fect has a higher efficiency with lower levels being required. Its excellent reproducibility in combination with its high level of efficiency reduced optimisation work to a minimum. Because of its low toxicity, a wide range of cell lines can be transfected efficiently.
An efficient triple-cotransfection using ROTI®Fect is described in Ufartes, Schlüter, Kaulfuss and Seipp (2005) Biospektrum 4: 464-5.
- Highly efficient reproduction of DNA in mammal cells
- Excellent reproducibility
- High and wide operating plateau
- No inhibition through serum
- Low cell toxicity
- Successful with different cell lines
- Excellent transfection results
siRNA: CEM, COS-7, ES-D3,HeLa, HL60, LNCaP, OVCAR3, PC3, SW620
Primary cells: pAML, pHDF, pMEF, pRHC, pSMC
Cell lines: 143B, 293-T, A-293, A-431, AM-C6SC8, BHK, BV-2, C2C12, C6, CHO, CHO-DHFR, COS-1, COS-7, CRFK, Colo-205, DU-145, ECV-304, EMC, F98 (glioma), GD25-ß1, HBL 100, HCT-116, HCT-15, HEK293, HeLa, HeLa-S3, Hep-3B, Hep-G2, HOSE, HSG, HT-22, HT-29, HUVEC, JURKAT, LLC-PK1, LS174T, LoVo, MCF-7, MDCK, MeWo, MV3, NIH-3T3, NS20Y, OK, P815, PC3, PT-11, Rcho-1, SAOS, SH-SY5Y, SK-MEL-28, SKOV-3, SM10, SW-480, THP-1, tsA201, U87 (glioma), Vero 76, et al.
Transfection of a variety of cell lines with ROTI®Fect and other commercially available transfectants.
Transfection was performed in 24wells using 0.25 µg DNA each, with 1.5 µl ROTI®Fect or acc. to instructions given by the manufacturers. Luciferase activity was measured 48 hours post transfection. Data of cells transfected with ROTI®Fect was set to 100 %.
- Subtotal: 0.00
|Order No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|P001.1||0.2 ml||plastic||1 x 0,2 ml||€79.45||
|P001.2||0.5 ml||plastic||1 x 0,5 ml||€170.40||
|P001.3||1 ml||plastic||1x 1 ml||€266.10||
|P001.4||5 ml||plastic||5 x 1 ml||€1,159.95||
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