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ROTI®Aqua-P/C/I, 250 ml

ready-to-use, for RNA extraction
Icon_EVE-EUD Please consider that this product might be liable to some legal sanctions as for example to obtain an end user declaration or the prohibition of some applications. For details please do contact your counterpart in our sales department!
Icon_ready-to-use Ready-to-use products and reagents
Examples of effect: Damage metals and burn body tissues; may cause serious eye damage. Safety: Avoid contact; wear safety spectacles and gloves. In the event of contact with eyes and skin, rinse with water.
Examples of effect: In smaller quantities, lead to immediate serious damage to health or death. Safety: Do not inhale, touch or swallow. Wear work safety equipment. Call a poison control centre or doctor immediately. Recovery position.
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
Examples of effect: Are harmful to aquatic organisms, toxic or highly toxic, acute or with long-term effects. Safety: To be disposed of as hazardous waste only, do not allow into the environment under any circumstances.
Danger
H301+H331-H312-H314-H341-H351-H361d-H372-H411
i toxic if swallowed or if inhaled, harmful in contact with skin, causes severe skin burns and eye damage, suspected of causing genetic defects, suspected of causing cancer, suspected of damaging the unborn child, causes damage to organs through prolonged or repeated exposure, toxic to aquatic life with long lasting effects
P201 P280 P304+P340 P305+P351+P338 P308+P313
i obtain special instructions before use, wear protective clothing/eye protection/face protection, IF INHALED: Remove person to fresh air and keep comfortable for breathing, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, IF exposed or concerned: Get medical advice/attention
Pack Qty.
Pack.
Phenol solutions
Boiling point (bp) >65 °C
Storage temp. +4 °C
ADR 6.1 II
WGK 3
UN-Nr. 2821

Redistilled, in water saturated phenol, chloroform and isoamyl alcohol at a ratio of 25:24:1, pH 4,5-5.
Store in the dark!

ROTI®Aqua-P/C/I is the optimal choice for RNA isolation and other procedures where a low ...
Product details
€52.60 /Pack Qty.

€40.70/Pack Qty.  Campaign price!

excl. VAT. | 250 ml per Pack Qty.

Art. No. X985.1

In stock
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Product details


  • Reduce exposure to toxic chemicals
  • Prepared from phenol of highest purity
  • Packed under argon for maximum stability
  • Successfully tried and tested in many research laboratories


Please note our Technical Information Brochure on the Internet next to the products:

Phenolic DNA Isolation
Background and protocol


Mechanism

MechanismPhenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses.
For distinct phase separation, ROTI®Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl.

Draw solution from lower phase. Do not shake before use!



ROTI®Aqua-P/C/I ready-to-use, for RNA extraction

ROTI®Aqua-P/C/I is the optimal choice for RNA isolation and other procedures where a low phenol pH value is desired.
The low pH results in interphase enrichment of the DNA, reducing DNA contamination in the RNA eluate.


Mechanism

Addition of 1 volume ROTI®Aqua-P/C/I and thorough mixing. Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. The acid pH-value causes a depletion of the DNA, so that only little contaminating DNA is isolated. Following centrifugation, the RNA containing solution may be retrieved as upper phase.
For distinct phase separation, ROTI®Aqua-P/C/I needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 12 drops of 1 N HCl.



ROTI®Aqua-P/C/I
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Order No. Pack Qty. Pack. Price Quantity
X985.1 250 ml glass €40.70
X985.2 500 ml glass €62.75
X985.3 100 ml glass €23.35
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