ROTI®Aqua-P/C/I, 250 ml
Storage temp. +4 °C
ADR 6.1 II
Store in the dark!
€40.70/Pack Qty. Campaign price!
excl. VAT. | 250 ml per Pack Qty.
Art. No. X985.1
Now recurring orders conveniently delivered as a subscription!
With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!How it works:
Put all products for your subscription in the desired quantity in the basket.
In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.
Set Starty point and interval for your subscription and submit order!
By the way: Through your account you can customize or delete your subscriptions anytime.
Do you have a question about this product?
- Reduce exposure to toxic chemicals
- Prepared from phenol of highest purity
- Packed under argon for maximum stability
- Successfully tried and tested in many research laboratories
Please note our Technical Information Brochure on the Internet next to the products:
Phenolic DNA Isolation
Background and protocol
MechanismPhenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses.
For distinct phase separation, ROTI®Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl.
Draw solution from lower phase. Do not shake before use!
ROTI®Aqua-P/C/I ready-to-use, for RNA extraction
ROTI®Aqua-P/C/I is the optimal choice for RNA isolation and other procedures where a low phenol pH value is desired.
The low pH results in interphase enrichment of the DNA, reducing DNA contamination in the RNA eluate.
Addition of 1 volume ROTI®Aqua-P/C/I and thorough mixing. Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. The acid pH-value causes a depletion of the DNA, so that only little contaminating DNA is isolated. Following centrifugation, the RNA containing solution may be retrieved as upper phase.
For distinct phase separation, ROTI®Aqua-P/C/I needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 12 drops of 1 N HCl.
- Subtotal: 0.00
|Order No.||Pack Qty.||Pack.||Price||Quantity|
Available at short notice
Delivery date currently unknown