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ROTI®Aqua-Phenol, 500 ml

for RNA extraction
Icon_EVE-EUD Please consider that this product might be liable to some legal sanctions as for example to obtain an end user declaration or the prohibition of some applications. For details please do contact your counterpart in our sales department!
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Examples of effect: Damage metals and burn body tissues; may cause serious eye damage. Safety: Avoid contact; wear safety spectacles and gloves. In the event of contact with eyes and skin, rinse with water.
Examples of effect: In smaller quantities, lead to immediate serious damage to health or death. Safety: Do not inhale, touch or swallow. Wear work safety equipment. Call a poison control centre or doctor immediately. Recovery position.
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
Examples of effect: Are harmful to aquatic organisms, toxic or highly toxic, acute or with long-term effects. Safety: To be disposed of as hazardous waste only, do not allow into the environment under any circumstances.
Danger
H301+H311+H331-H314-H341-H373-H411
i toxic if swallowed, in contact with skin or if inhaled, causes severe skin burns and eye damage, suspected of causing genetic defects, may cause damage to organs through prolonged or repeated exposure, toxic to aquatic life with long lasting effects
P201 P280 P304+P340 P305+P351+P338 P310
i obtain special instructions before use, wear protective gloves/protective clothing/eye protection/face protection, IF INHALED: Remove person to fresh air and keep comfortable for breathing, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, immediately call a POISON CENTER/doctor
Pack Qty.
Pack.
Phenol solutions
Density (D) 1,03 g/cm³
Flash point (flp) 81 °C
Storage temp. +4 °C
ADR 6.1 III
WGK 2
UN-Nr. 2821

Redistilled, in water saturated phenol, pH 4,5-5.
Draw solution from lower phase. Do not shake before use!
Covered with water. Store in the dark!

ROTI®Aqua Phenol is perfectly suited for RNA isolation and other procedures, requirin ...
Product details

€68.70/Pack Qty. 

excl. VAT. | 500 ml per Pack Qty.

Art. No. A980.3

In stock
Delivery fast, simple and reliable!
from 6 Pack Qty. €65.26/Pack Qty.
from 24 Pack Qty. €61.83/Pack Qty.
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Please note our Technical Information Brochure on the Internet next to the products:

Phenolic DNA Isolation
Background and protocol

Mechanism

Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses.
For distinct phase separation, ROTI®Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl.

Draw solution from lower phase. Do not shake before use!


ROTI®Aqua-Phenol for RNA extraction

ROTI®Aqua Phenol is perfectly suited for RNA isolation and other procedures, requiring a low phenol pH value. The low pH results in interphase enrichment of the DNA, reducing DNA contamination in the RNA eluate.

Also ideal for self-adjustment of the pH-value. If required, buffer solution (e.g. TE buffer) may be added directly into the bottle, as ROTI®Aqua-Phenol comes in the following bottle sizes:
A980.2: 100 ml in a 250 ml bottle
A980.1: 250 ml in a 500 ml bottle
A980.3: 500 ml in a 1 l bottle


Mechanism

Addition of 1 volume ROTI®Aqua-Phenol and thorough mixing. Phenol leads to denaturation of proteins which accumulate in the interphase. The acid pH-value causes a depletion of the DNA, so that only little contaminating DNA is isolated. Following centrifugation, the RNA containing solution may be retrieved as upper phase.
For distinct phase separation, ROTI®Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 12 drops of 1 N HCl.



ROTI®Aqua-Phenol
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Order No. Pack Qty. Pack. Price Quantity
A980.1 250 ml glass €44.00
A980.2 100 ml glass €23.15
A980.3 500 ml glass €68.70
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