ROTI®Aqua-Phenol, 500 ml
Please consider that this product might be liable to some legal sanctions as for example to obtain an end user declaration or the prohibition of some applications. For details please do contact your counterpart in our sales department!
Ready-to-use products and reagents
Flash point (flp) 81 °C
Storage temp. +4 °C
ADR 6.1 III
WGK 2
UN-Nr. 2821
Draw solution from lower phase. Do not shake before use!
Covered with water. Store in the dark!
€68.70/Pack Qty.
excl. VAT. | 500 ml per Pack Qty.
Art. No. A980.3
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Product details
Phenolic DNA Isolation
Background and protocol
For distinct phase separation, ROTI®Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl.
Draw solution from lower phase. Do not shake before use!
ROTI®Aqua-Phenol for RNA extraction
ROTI®Aqua Phenol is perfectly suited for RNA isolation and other procedures, requiring a low phenol pH value. The low pH results in interphase enrichment of the DNA, reducing DNA contamination in the RNA eluate.
Also ideal for self-adjustment of the pH-value. If required, buffer solution (e.g. TE buffer) may be added directly into the bottle, as ROTI®Aqua-Phenol comes in the following bottle sizes:
A980.2: 100 ml in a 250 ml bottle
A980.1: 250 ml in a 500 ml bottle
A980.3: 500 ml in a 1 l bottle
Addition of 1 volume ROTI®Aqua-Phenol and thorough mixing. Phenol leads to denaturation of proteins which accumulate in the interphase. The acid pH-value causes a depletion of the DNA, so that only little contaminating DNA is isolated. Following centrifugation, the RNA containing solution may be retrieved as upper phase.
For distinct phase separation, ROTI®Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 12 drops of 1 N HCl.
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| Order No. | Pack Qty. | Pack. | Price | Quantity | ||
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| A980.1 | 250 ml | glass | €44.00 |
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| A980.2 | 100 ml | glass | €23.15 |
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| A980.3 | 500 ml | glass | €68.70 |
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