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ROTI®Sep 1077 human/tube

CELLPURE® sterile, ready-to-use, for density gradient centrifugation
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Icon_ready-to-use Ready-to-use products and reagents
Sterile
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Lymphocyte separation medium, Polysucrose solution
Molar mass (M) ~400000 g/mol
Density (D) 1,078 g/cm³
Storage temp. +4 °C
WGK 1

Polysucrose based medium for separation of lymphocytes/PBMC from human whole blood. Density: 1,077 g/ml
15 ml prefilled in 50 ml tubes.
Long-term storage at approx. 4 °C recommended. Short-term storage at room temperature possible. Storage protected from light.

Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from human blood by density gradient centrifugation.
Density 1.077 g/ml.
Best alternative to Ficoll®.
Product details

€806.25/Pack Qty. 

excl. VAT. | 25 unit(s) per Pack Qty.

Art. No. 0634.1

In production
from 6 Pack Qty. €765.94/Pack Qty.
from 24 Pack Qty. €725.62/Pack Qty.

Product details

Sterile

Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from human blood by density gradient centrifugation.
Density 1.077 g/ml.
Best alternative to Ficoll®.

  • Optimised for isolation of lymphocytes from non-coagulated human whole blood
  • Produces distinct, compact layers
  • Results in high recovery rates of viable cells
  • Maintains the representative ratio of B- to T lymphocytes
  • May be directly applied to all protocols using Ficoll®

ROTI®Sep 1077 human excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ratio of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range.

Suitable for application with human non-coagulated peripheral blood. In all respective protocols originally using Ficoll® this reagent may be replaced by ROTI®Sep 1077 human without any adjustment.



ROTI®Sep 1077 human/tube CELLPURE® sterile, ready-to-use, for density gradient centrifugation

Prefilled in 50 ml centrifugation tubes, 15 ml each.


With permeable membrane for easy pipetting of diluted blood.


0634_Anwendung


Not a medical device / Not an IVD product
ROTI®Sep 1077 human/tube
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Art. No. Pack Qty. Pack. Packaging Price Quantity
0634.1 25 unit(s) cardboard 25 x 50 ml

€806.25

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Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

Appearance of solutionclear, colourless
Density1,077±0,001 g/ml
pH value7,15±0,35
Osmolality315±25 mOsm/kg
Endotoxinstested
Sterilitycomplies