Jump to content Jump to navigation menu

ROTI®Sep 1077 human, 100 ml

CELLPURE® sterile, ready-to-use, for density gradient centrifugation
Icon_Steril Sterile products
Icon_ready-to-use Ready-to-use products and reagents
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
Danger
H317-H334
i may cause an allergic skin reaction, may cause allergy or asthma symptoms or breathing difficulties if inhaled
P261 P302+P352 P333+P313
i avoid breathing mist/vapours, IF ON SKIN: Wash with plenty of water, if skin irritation or rash occurs: Get medical advice/attention
Sterile
Pack Qty.
Pack.
Lymphocyte separation medium, Polysucrose solution
Molar mass (M) ~400000 g/mol
Density (D) 1,08 g/cm³
Boiling point (bp) 100 °C
Storage temp. +4 °C
WGK 1

Polysucrose based medium for separation of lymphocytes/PBMC from human whole blood. Density: 1,077 g/ml
Long-term storage at approx. 4 °C recommended. Short-term storage at room temperature possible. Storage protected from light.

Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from human blood by density gradient centrifugation.
Density 1.077 g/ml.
Best alternative to Ficoll®.
Product details
€46.80 /Pack Qty.

€36.40/Pack Qty.  Campaign price!

excl. VAT. | 100 ml per Pack Qty.

Art. No. 0642.1

In stock
Delivery fast, simple and reliable!
Carl Roth subscription service

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:
1

Put all products for your subscription in the desired quantity in the basket.

2

In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.

3

Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Product details

Sterile

Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from human blood by density gradient centrifugation.
Density 1.077 g/ml.
Best alternative to Ficoll®.

  • Optimised for isolation of lymphocytes from non-coagulated human whole blood
  • Produces distinct, compact layers
  • Results in high recovery rates of viable cells
  • Maintains the representative ratio of B- to T lymphocytes
  • May be directly applied to all protocols using Ficoll®

ROTI®Sep 1077 human excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ratio of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range.

Suitable for application with human non-coagulated peripheral blood. In all respective protocols originally using Ficoll® this reagent may be replaced by ROTI®Sep 1077 human without any adjustment.



ROTI®Sep 1077 human CELLPURE® sterile, ready-to-use, for density gradient centrifugation

For research use only. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro diagnostics.

0642_02
The figure depicts two specimens prepared with heparinic blood obtained from venous puncture. Density gradient centrifugation of 6 ml diluted whole blood (dilution in PBS 1:1.5), with 3 ml of ROTI®Sep 1077 human, 10 mins., 1000 g, no break.


Not a medical device / Not an IVD product
ROTI®Sep 1077 human
Selected quantity:   0
  1. Subtotal:  0.00
Order No. Pack Qty. Pack. Price Quantity
0642.1 100 ml plastic €36.40
0642.2 500 ml plastic €97.70
In stock
Available at short notice
Not available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Appearance of solutionclear, colourless
Density1,077 ±0,001 g/ml
pH value7,0 ±0,5
Osmolality325 ±35 mOsm/kg
Sterilitycomplies
Endotoxinstested
For research use only. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro diagnostics.

0 Reviews

0.0 out of five stars