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HPLC column NUCLEODUR® 300-5 C4 ec, 250 mm, 4 mm

From  Macherey-Nagel
Material: stainless steel.
Particle size
Column length
Column inner diameter


Wide pore RP phase for daily routine analysis. The medium density butyl modification is based on high purity NUCLEODUR® silica gel and is often used for the separation of biomolecules.
Product details

€462.25/Pack Qty. 

excl. VAT. | 1 unit(s) per Pack Qty.

Art. No. 1HKK.1

Available
Ready for dispatch in 3-4 weeks
from 6 Pack Qty. €439.14/Pack Qty.
from 24 Pack Qty. €416.02/Pack Qty.

Product details

Material: stainless steel.

Wide pore RP phase for daily routine analysis. The medium density butyl modification is based on high purity NUCLEODUR® silica gel and is often used for the separation of biomolecules.


Butyl (C4) modifications have a lower retention for hydrophobic compounds compared to C18 (octadecyl silane, ODS) or C8 (octyl, MOS) modifications.

Properties:
  • Butyl phase, endcapped
  • Carbon content: 2.5 %
  • Pore size: 300 Å
  • pH stability: 1-9
  • Eluent on column: acetonitrile/water

Similar phases:
Jupiter® C4; ACE® C4.


Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


For protection of your main HPLC column from contamination, it is recommended to use guard columns. These short columns are placed into a guard column holder. The guard column holder in combination with a guard column inside is installed between the injector and the HPLC column. This installation prevents chemical impurities to reach the main column. Because a contamination could form an irreversible bond with the column packing material (stationary phase) and this can result in shorter column life. If you want to prolong the lifetime of your main column, then these short columns can help you.


Further HPLC columns from Macherey-Nagel available on request.


HPLC Columns NUCLEODUR® ec

Non-polar phases for routine analysis

Standard RP phase (110 Å) for daily routine analysis and up-scaling to preparative HPLC. Also available wide-pore (300 Å) for separation of biomolecules in analytical columns.

Properties:

  • Cost-effective and reliable
  • Long-life columns
  • For all standard routine applications in modern RP-chromatography


Recommended application(s):

  • Pore size 100 Å:
    Basic, neutral and acidic pharmaceuticals, derivatised amino acids, pesticides, fat-soluble vitamins, aldehydes and ketones, phenolic compounds.
  • Pore size 300 Å:
    Biological macromolecules, such as proteins or peptides.
  • USP L1 (C18) · USP L7 (C8) · USP L26 (C4)



HPLC column NUCLEODUR® 300-5 C4 ec 

Technical Information
Particle size 5 µm
Column length 250 mm
Column inner diameter 4 mm
Column volume 3.15 ml
Endcapped yes 
Eluent in column Acetonitrile - Water 
Phase 300-5 C4 ec 
Surface chemistry Medium density monomeric butyl (C4) wide-pore modification 
USP listing L26 
Separation principle Reversed phase (RP) 
Column shape Analytical separation column 
Column type HPLC column 
Particle type Fully porous particles (FPP) 
Specific surface according to BET 100 m²/g
Pore volume 0.9 ml/g
Pore size 300 Å
Temperature stability 60 °C 
Pressure stability 450 bar 
pH stability 1,0-9,0 
Recommended application(s) Biological macromolecules, Peptides, Proteins 
Similar phases Jupiter® C4 
ACE® C4 
HPLC column NUCLEODUR® 300-5 C4 ec
Selected quantity:   0
  1. Subtotal:  0.00
Art. No. Particle size Column length Column inner diameter Pack Qty. Price Quantity
1HKE.1 5 µm 150 mm 4 mm 1 unit(s)

€379.50

1HKL.1 5 µm 250 mm 4,6 mm 1 unit(s)

€488.05

1HKK.1 5 µm 250 mm 4 mm 1 unit(s)

€462.25

1HKH.1 5 µm 150 mm 4,6 mm 1 unit(s)

€401.00

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Downloads / MSDS

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General information

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


NUCLEODUR® Columns for HPLC

NUCLEODUR® as a state-of-the-art silica is the ideal base material for modern HPLC phases. We offer two phases NUCLEODUR® Gravity which is ideal for sophisticated analytical separations and NUCLEODUR® ec suitable for routine HPLC analysis.