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HPLC column NUCLEODUR® C18 Gravity 5 µm, 250 mm, 4.6 mm

From  Macherey-Nagel
Material: stainless steel.
Particle size
Column length
Column inner diameter

Product details

€790.15/Pack Qty. 

excl. VAT. | 1 unit(s) per Pack Qty.

Art. No. KK91.1

Ready for dispatch in 3-4 weeks
from 6 Pack Qty. €750.64/Pack Qty.
from 24 Pack Qty. €711.14/Pack Qty.

Product details

Material: stainless steel.

  • Octadecyl phase, multi-endcapped
  • Carbon content: 18.0 %
  • Pore size: 110 Å
  • pH stability: 1-11
  • Eluent on column: acetonitrile/water

Pharmaceuticals, e.g. analgesics, antiinflammatory drugs, antidepressants, herbicides, immunosuppressants, USP L1, suitable for LC-MS.

Similar phases:
Zorbax® Extend C18, Synergi™ Max RP, Phenomenex Luna® C18 (2), Waters Xterra® RP18 / MS C18, Inertsil® ODS III, Purospher® RP-18, Star RP-18.

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!

For protection of your main HPLC column from contamination, it is recommended to use guard columns. These short columns are placed into a guard column holder. The guard column holder in combination with a guard column inside is installed between the injector and the HPLC column. This installation prevents chemical impurities to reach the main column. Because a contamination could form an irreversible bond with the column packing material (stationary phase) and this can result in shorter column life. If you want to prolong the lifetime of your main column, then these short columns can help you.

Further HPLC columns from Macherey-Nagel available on request.

HPLC Columns NUCLEODUR® “Gravity”

The ideal phase for overall sophisticated analytical separations

This high density phase with its endcapping suppresses any unwanted polar interactions between the silica surface and the sample, which makes “Gravity” particularly suitable for the separation of basic and other ionizable analytes.


  • Suitable for LC/MS and HPLC at pH extremes (pH 1-11)
  • Ideal for method development
  • Superior base deactivation
  • Multi-Endcapping

Recommended application(s):

  • In general, compounds with ionisable functional groups such as basic pharmaceuticals and pesticides e.g. analgesics, anti-inflammatories, antidepressants, herbicides, phytopharmaceuticals, immunosuppressants.
  • USP L1 (C18) · USP L7 (C8)

HPLC column NUCLEODUR® C18 Gravity 5 µm 

Technical Information
Particle size 5 µm
Column length 250 mm
Column inner diameter 4.6 mm
Column volume 4.15 ml
Endcapped yes 
Eluent in column Acetonitrile - Water 
Phase C18 Gravity 
Surface chemistry Base deactivated (BDS), High density octadecyl (C18, ODS) modification 
USP listing L1 
Separation principle Reversed phase (RP) 
Column shape Analytical separation column 
Column type HPLC column 
Particle type Fully porous particles (FPP) 
Specific surface according to BET 340 m²/g
Pore volume 0.9 ml/g
Pore size 110 Å
Temperature stability 60 °C 
Pressure stability 600 bar 
pH stability 1,0-11,0 
Recommended application(s) Analgesics, Antidepressants, Antiinflammatory drugs, Applications at pH extremes (1-11), Herbicides, Ideal for method development, Immunosuppressants, Pharmaceuticals, Phytopharmaceuticals 
Similar phases NUCLEOSIL® C18 HD 
Zorbax® Extend C18 
Synergi™ Max RP 
Inertsil® ODS III 
Luna® C18(2) 
Xterra® RP18 / MS C18 
Purospher® Star RP-18 
Hypersil™ BDS 
HPLC column NUCLEODUR® C18 Gravity 5 µm
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Art. No. Particle size Column length Column inner diameter Pack Qty. Price Quantity
KK89.1 5 µm 250 mm 4 mm 1 unit(s)


KK90.1 5 µm 150 mm 4,6 mm 1 unit(s)


KK91.1 5 µm 250 mm 4,6 mm 1 unit(s)


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Downloads / MSDS

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General information

Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!


NUCLEODUR® as a state-of-the-art silica is the ideal base material for modern HPLC phases. We offer two phases NUCLEODUR® Gravity which is ideal for sophisticated analytical separations and NUCLEODUR® ec suitable for routine HPLC analysis.

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