Technical Data Sheet
Size Exclusion Chromatography Columns ROTI®Dex-25 Medium Grav L, 25 unit(s)
Ready-to-use products and reagents
For inquiries and further information, please contact our sales team
For desalting and buffer exchange of protein and nucleic acid solutions.
€314.45/Pack Qty.
excl. VAT. | 25 unit(s) per Pack Qty.
Art. No. 21C1.1
Product details
Prepacked ROTI®Dex-Grav columns for size exclusion chromatography by gravity, for fast and efficient removal of unwanted low molecular weight factors, such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions.
The purification is based on a spherical porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin.
In size exclusion chromatography molecules are separated according to their size in solution. Smaller molecules pass through the column much slower than larger molecules.
In contrast to other chromatography methods, no interactions occur here between the analyte and the stationary phase of the column. This provides the ideal basis for the purification and analysis of intact proteins.
- Sample preparation in just a few minutes
- Desalting, removal of impurities and buffer exchange in a single step
- No interaction between analyte and medium
- High selectivity, high resolution and high yield (> 95%)
- Also suitable for small sample volumes
- High chemical stability and therefore compatible with many organic and other solvents
Size Exclusion Chromatography Columns ROTI®Dex-25 Medium Grav L
The column bed consists of ROTI®Dex-25 Medium (Art. No. 21A5), pre-swollen in deionised water.
- Oligonucleotides > 10 bp
- Globular proteins > 5 kDa
- Particles > 2 nm
Column size L is recommended for sample volumes of 2.5 ml.
Detailed information on the application can be found in our Technical Information Brochure and in our Instructions for Use.
| Version | Grav L |
| Matrix | Cross-linked dextran |
| MWCO |
5 kDa 10 bp |
| Particle size |
Dry: 50-150 µm Wet: 85-260 µm |
| Storage buffer | Deionized water with 0.15% ProClin® 150 |
| Sample volume | 2.5 ml |
| Elution volume | 3.5 ml |
- Subtotal: 0.00
| Art. No. | Version | Pack Qty. | Price | Quantity | |
|---|---|---|---|---|---|
| 21C1.1 | Grav L | 25 unit(s) |
€314.45 |
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In stock
Available (note delivery time)
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
Further attractive products to complete your chromatography laboratory can be found on our Chromatography page!
The ROTI®Dex ready-to-use columns at a glance
| Application | Order No. | Matrix | Particle size (wet) | Gel bed volume | Sample volume | MWCO | Pack Qty. |
| Spin | 21C4.1 | ROTI®Dex 25 Medium | 85-260 µm | 0.5 ml | 2-100 µl (50 µl optimal) | Proteins >5 kDa, Oligonucleotides >10 bp, Nanoparticles >2 nm |
25 Pcs |
| 21C5.1 | ROTI®Dex 50 Medium | 100-300 µm | 0.5 ml | 2-100 µl (50 µl optimal) | Proteins >25 kDa, Oligonucleotides >20 bp, Nanoparticles >4 nm |
25 Pcs | |
| Grav | 21AX.1 | ROTI®Dex 25 Medium | 85-260 µm | 1.78 ml | 0.15-0.3 ml | Proteins >5 kDa, Oligonucleotides >10 bp, Nanoparticles >2 nm |
50 Pcs |
| 21AY.1 | 2.75 ml | 0.5 ml | 50 Pcs | ||||
| 21C0.1 | 4.31 ml | 1 ml | 50 Pcs | ||||
| 21C1.1 | 10.37 ml | 2.5 ml | 25 Pcs | ||||
| 21C2.1 | 17.2 ml | 5 ml | 10 Pcs | ||||
| 21C3.1 | 34.21 ml | 10 ml | 10 Pcs | ||||
| FPLC | 21C6.1 | ROTI®Dex 25 Superfine | 40-110 μm | 1 ml | 0.05-0.3 ml | Proteins >5 kDa, Oligonucleotides >10 bp, Nanoparticles >2 nm |
5 Pcs |
| 21C6.2 | 1 ml | 0.05-0.3 ml | 100 Pcs | ||||
| 21C7.1 | 5 ml | 0.1-1.5 ml | 5 Pcs | ||||
| 21C7.2 | 5 ml | 0.1-1.5 ml | 25 Pcs | ||||
| 21C7.3 | 5 ml | 0.1-1.5 ml | 100 Pcs (4x25) |
Certificates of Analysis
Type analysis
| Flow rate (2.5 mL water) | ≤85 s |
| Salt removal (Conductivity of 3.5 mL eluate from a 2.5 mL 0.8M NaCl application) | ≤1000 µS/cm |
| Dextran Blue recovery yield (Yield of Dextran blue in 3.5 mL eluate from a 2.5 mL solution of 1 mg/mL Dextran Blue application) | ≥85 % |