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Eva Green® Fluorescent Dye

100x konz., for electrophoresis
Pack Qty.
Pack.
Ethidium bromide substitutes
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 3

For fluorescent staining of nucleic acids in agarose- and polyacrylamide gels.
500 µl is sufficient for running and staining of approx. 500 lanes.

Type analysis

€90.85/Pack Qty. 

excl. VAT. | 500 µl per Pack Qty.

Art. No. 8905.1

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Product details


  • Direct addition to DNA sample
  • For excitation with UV light and blue light
  • Non-toxic alternative for ethidium bromide


Eva Green® Fluorescent Dye 100x konz., for electrophoresis

EvaGreen® is a staining reagent for detection of double stranded nucleic acid in agarose- and polyacrylamide gels. The dye is added directly to the DNA/Loading buffer mixture and then loaded onto the gel. EvaGreen® bounds to nucleic acids emitting a brightly coloured fluorescence. The sensitivity reaches up to 0.2 ng per band.
EvaGreen® is an intercalating fluorescent dye, but due to its low membrane permeability it is non mutagenic, non-toxic, and environment friendly. EvaGreen® has been developed specifically for gentle DNA analysis. DNA integrity is not influenced; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligations, PCR, transformation, transfection etc. without interfering effects.


EvaGreen® can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTI®PROfessional runVIEW, Art. No. 4849.1). The gels may be documented by all usual broadband ethidium bromide- or SYBR® Green foto filters.


Wavelength

Excitation maximum (bound to DNA): approx. 320 nm and 470-500 nm
Emission maximum (bound to DNA): 525 nm



Technical Information
Excitation max. (DNA-bound) ca. 320  nm
470–500  nm
Emission max. (DNA-bound) 525 nm  
Eva Green® Fluorescent Dye
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8905.1 500 µl plastic €90.85
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Type analysis

Appearanceorange, clear solution

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