ROTI®GelStain Red, 1 ml, 1 x 1 ml
1 ml is sufficient for staining of approx. 600 minigels with 30 ml agarose volume each.
€79.35/Pack Qty. Campaign price!
excl. VAT. | 1 ml per Pack Qty.
Art. No. 0984.1
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Non-toxic and non-carcinogenic dyes for fluorescent staining of nucleic acids in agarose gels and polyacrylamide gels. Highly sensitive and applied just like ethidium bromide.
The ROTI®GelStain dyes are used as additives to the gel solution and the running buffer, partly also post-run staining is possible. Both ROTI®GelStain dyes can be excited by UV light, additionally, ROTI®GelStain Red may be excited by blue light. Resulting signals may be detected by ethidium bromide broadband photo filters.
ROTI®GelStain Red 20 000x conc., ready-to-use
ROTI®-GelStain Red is a versatile staining reagent for detection of all nucleic acids in agarose and polyacrylamide gels.
Suitable for staining of dsDNA, ssDNA and RNA.
- Red fluorescence
- Alternative to ethidium bromide, non-toxic, non-mutagen
- Detection of > 0.3 ng nucleic acid per band
- Usuable with the same filters as ethidium bromide
- Excitation via UV light (310 nm) and blue light (540 nm)
- Compatible with all usual down-stream applications
ROTI®-GelStain Red is a red fluorescent dye that can be used like ethidium bromide for staining in gel (addition to gel solution). Due to the low membrane permeability of the dye ROTI®-GelStain Red is non-mutagenic and non-toxic. It is suitable for staining of dsDNA, ssDNA and RNA.
ROTI®-GelStain detects up to 0.3 ng per band, making it more sensitive than ethidium bromide.
Bound to nucleic acid it emits a brightly red fluorescence that can be documented by all usual ethidium bromide foto filters. ROTI®-GelStain Red is compatible with all usual down-stream applications.
Excitation maximum (bound to DNA): approx.310 nm and 540 nm
Emission maximum (bound to DNA): 630 nm
- Subtotal: 0.00
|Order No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|0984.1||1 ml||plastic||1 x 1 ml||
|0984.2||5 ml||plastic||5 x 1 ml||
Delivery date currently unknown
Summary Staining of Nucleic Acid in Gels
|Ethidium bromide solution||0.5-5 ng/band||Standard fluorescent staining||254-330 nm, 500 nm UV- and blue light|
|SYBR® Green DNA dye||0.01 ng/band||Non-toxic, green fluorescent staining||254 nm and 495 nm UV- and blue light|
|ROTI®GelStain||0.3 ng/band||Non-toxic, green fluorescent staining||302 nm and 490 nm UV- and blue light|
|ROTI®GelStain Red||0.3 ng/band||Non-toxic, red fluorescent staining||310 nm, 540 nm UV- and blue light|
|ROTI®Methylene blue staining concentrate||10 ng/band||Reversible, non-toxic blue staining||White light|
|ROTI®Black N||<0.1 ng/band||Silver staining of DNA polyacrylic amide gels||White light|
|Appearance||red, transparent liquid, no precipitation|
|Staining of DNA in agarose gels||complies|
|Staining of DNA in PA gels||complies|