ROTI®GelStain, 1 ml, 1 x 1 ml
Boiling point (bp) ~100 °C
Storage temp. +4 °C
1 ml is sufficient for staining of approx. 600 minigels with 30 ml agarose volume each.
excl. VAT. | 1 ml per Pack Qty.
Art. No. 3865.1
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Non-toxic and non-carcinogenic dyes for fluorescent staining of nucleic acids in agarose gels and polyacrylamide gels. Highly sensitive and applied just like ethidium bromide.
The ROTI®GelStain dyes are used as additives to the gel solution and the running buffer, partly also post-run staining is possible. Both ROTI®GelStain dyes can be excited by UV light, additionally, ROTI®GelStain Red may be excited by blue light. Resulting signals may be detected by ethidium bromide broadband photo filters.
ROTI®GelStain ready-to-use, for electrophoresis
ROTI®GelStain is a versatile staining reagent for detection of all nucleic acids in agarose gels.
- Green fluorescence
- Alternative for ethidium bromide, non-toxic, non-mutagen
- For dsDNA, ssDNA and RNA
- Nearly as sensitive as ethidium bromide (approx. 0.2 ng / band)
- For use with common broad-band ethidium bromide photo filters
- Excitation via UV light (302 nm) and blue light (490 nm)
- Compatible with all usual down-stream applications
The dye may be used exactly in the same way as ethidium bromide - for staining in-gel, staining through running buffer, or post-run staining -, and may replace this toxic and potentially mutagen reagent completely.
ROTI®-GelStain detects up to 1,5 ng/mm2 nucleic acid, which is approx. 0.2 ng per band, and is, therefore, nearly as sensitive as ethidium bromide.
When bound to nucleic acids in agarose gels, ROTI®-GelStain emits a brightly green fluorescence that may be documented by all usual broadband ethidium bromide or SYBR® Green foto filters.
ROTI®-GelStain is compatible with all standard down-stream applications and has been successfully tested for use with gel extraction, ligation, transformation and transfection.
Amplicons of a mouse genotyping (left), and restriction digested DNA (right), respectively, separated in 2,5 and 1 % agarose ROTI®Garose NEEO with ROTI®GelStain (5 µl / 100 ml agarose). Pictures were taken using standard ethidium bromide photo filters.
With kind permission of Prof. R. Slany, Dept. of Genetics, Friedrich Alexander University, Erlangen.
Excitation maximum (bound to DNA): 302 nm (UV) and 490 nm (blue light)
Emission maximum (bound to DNA): 520 nm
|Max. excitation (DNA-bound)||302 nm (UV), 490 nm|
|Emissions max. (DNA-bound)||520 nm|
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|Order No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|3865.1||1 ml||plastic||1 x 1 ml||
|3865.2||5 ml||plastic||5 x 1 ml||
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