ROTI®Load DNAstain 1 SYBR® Green, 1.8 ml, 1 x 1.8 ml
Storage temp. -20 °C
Transport temp. ambient temp.
Contains: Tris, EDTA, bromophenol blue, xylencyanol, SYBR® Green, glycerol.
1.8 ml are sufficient for gel run and staining of approx. 1,800 lanes (5 µl sample + 1 µl loading buffer).
excl. VAT. | 1.8 ml per Pack Qty.
Art. No. 1CN5.1
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Loading bufer with the fluorescent dye SYBR® Green I for staining of double stranded nucleic acid in agarose and polyacrylamide gels.
- Alternative for ethidium bromide, non-toxic, non-mutagen
- 20 times more sensitive than ethidium bromide, up to 0.01 ng nucleic acid per band
- For use with common broad-band ethidium bromide photo filters
- Excitation possible via UV light (254 nm) and blue light (495 nm)
- Compatible with all usual down-stream applications.
ROTI®Load DNAstain SYBR® Green is easy to use and reduces significantly the time required for gel analysis. The contained fluorescent dye SYBR® Green I detects up to 0.01 ng per band, and is, therefore, 20 times more sensitive than ethidium bromide. DNA integrity is not influenced by SYBR® Green I; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligation, PCR, transformation, transfection etc. without interfering effects.
SYBR® Green I bounds to the minor groove of DNA and intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE®PROfessional runVIEW, Art. No. 4849.1).
When excited, nucleic acid-bound SYBR® Green I emits a brightly coloured green fluorescence (521 nm) that may be documented by all usual broadband ethidium bromide- or SYBR® Green foto filters.
The figure depicts 50 ng DNA per band, or 75 ng (1000 bp and 2.5 kb), respectively. Each lane has been loaded and concurrently stained using 1 µl ROTI®Load DNAstain SYBR® Green.
Excitation maximum (bound to DNA): 254 nm and 495 nm
Emission maximum (bound to DNA): 521 nm
ROTI®Load DNAstain 1 SYBR® Green 6x conc., ready-to-use
In order to cover the main size range needed in gel electrophoresis, ROTI®Load DNAstain 1 SYBR® Green contains bromophenol blue and xylene cyanol.
Glycerin is the standard reagent for increasing sample density.
Recommended for staining of dsDNA- and dsRNA-fragments of over 500 bp.
|Max. excitation (DNA-bound)||
|Amount of traces||ca. 1000/ml|
|Emissions max. (DNA-bound)||525 nm|
|Dye||Bromphenol blue, xylene cyanole, SYBR® Green|
|Incl. DNA staining||yes|
|Use||Simultaneous gel staining, bigger fragments|
- Subtotal: 0.00
|Order No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|1CN5.1||1.8 ml||plastic||1 x 1.8 ml||
|1CN5.2||9 ml||plastic||5 x 1.8 ml||
Delivery date currently unknown
|Appearance||dark blue solution|
|Each batch is functionally tested for its suitability in electrophoresis.|