Jump to content Jump to navigation menu

ROTI®Load DNAstain 1 SYBR® Green, 1.8 ml, 1 x 1.8 ml

6x conc., ready-to-use
Icon_ready-to-use Ready-to-use products and reagents
Pack Qty.
Density (D) ~1,12 g/cm³
Storage temp. -20 °C
Transport temp. ambient temp.

DNA-gel loading buffer with fluorescent DNA-dye SYBR® Green. For fragments >500 bp.
Contains: Tris, EDTA, bromophenol blue, xylencyanol, SYBR® Green, glycerol.
1.8 ml are sufficient for gel run and staining of approx. 1,800 lanes (5 µl sample + 1 µl loading buffer).

Loading bufer with the fluorescent dye SYBR® Green I for staining of double stranded nucleic acid in agarose and polyacrylamide gels.
Product details

€47.85/Pack Qty. 

excl. VAT. | 1.8 ml per Pack Qty.

Art. No. 1CN5.1

In stock
Delivery fast, simple and reliable!
from 6 Pack Qty. €45.46/Pack Qty.
from 24 Pack Qty. €43.06/Pack Qty.
Carl Roth subscription service

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:

Put all products for your subscription in the desired quantity in the basket.


In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.


Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Product details

Loading bufer with the fluorescent dye SYBR® Green I for staining of double stranded nucleic acid in agarose and polyacrylamide gels.

  • Alternative for ethidium bromide, non-toxic, non-mutagen
  • 20 times more sensitive than ethidium bromide, up to 0.01 ng nucleic acid per band
  • For use with common broad-band ethidium bromide photo filters
  • Excitation possible via UV light (254 nm) and blue light (495 nm)
  • Compatible with all usual down-stream applications.

ROTI®Load DNAstain SYBR® Green is easy to use and reduces significantly the time required for gel analysis. The contained fluorescent dye SYBR® Green I detects up to 0.01 ng per band, and is, therefore, 20 times more sensitive than ethidium bromide. DNA integrity is not influenced by SYBR® Green I; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligation, PCR, transformation, transfection etc. without interfering effects.

SYBR® Green I bounds to the minor groove of DNA and intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE®PROfessional runVIEW, Art. No. 4849.1).
When excited, nucleic acid-bound SYBR® Green I emits a brightly coloured green fluorescence (521 nm) that may be documented by all usual broadband ethidium bromide- or SYBR® Green foto filters.

The figure depicts 50 ng DNA per band, or 75 ng (1000 bp and 2.5 kb), respectively. Each lane has been loaded and concurrently stained using 1 µl ROTI®Load DNAstain SYBR® Green.


Excitation maximum (bound to DNA): 254 nm and 495 nm
Emission maximum (bound to DNA): 521 nm

ROTI®Load DNAstain 1 SYBR® Green 6x conc., ready-to-use
Directions for use

In order to cover the main size range needed in gel electrophoresis, ROTI®Load DNAstain 1 SYBR® Green contains bromophenol blue and xylene cyanol.

Glycerin is the standard reagent for increasing sample density.

Recommended for staining of dsDNA- and dsRNA-fragments of over 500 bp.

Technical Information
Max. excitation (DNA-bound) ~320 nm
470-500 nm
Amount of traces ca. 1000/ml 
Emissions max. (DNA-bound) 525 nm 
Dye Bromphenol blue, xylene cyanole, SYBR® Green 
Incl. DNA staining yes 
Sensitivity 0,01 ng/band
Use Simultaneous gel staining, bigger fragments 
ROTI®Load DNAstain 1 SYBR® Green
Selected quantity:   0
  1. Subtotal:  0.00
Order No. Pack Qty. Pack. Packaging Price Quantity
1CN5.1 1.8 ml plastic 1 x 1.8 ml


1CN5.2 9 ml plastic 5 x 1.8 ml


In stock
In procurement
Not available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS

Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Notes on the product

Appearancedark blue solution
Each batch is functionally tested for its suitability in electrophoresis.