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ROTI®Load DNAstain 3, 5 ml, 5 x 1 ml

6x konz., ready-to-use, DNase-free
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Icon_ready-to-use Ready-to-use products and reagents
Pack Qty.
Pack.
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Ethidium bromide substitutes
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1

DNA-gel loading buffer with fluorescent DNA-dye EvaGreen®. For fragments <500 bp.
Contains: Tris, EDTA, orange G, EvaGreen®.
1 ml is sufficient for running and staining of approx. 500 lanes.

Versatile reagent for gel loading and simultaneous staining of double stranded nucleic acid in agaro ...
Product details

€114.95/Pack Qty. 

excl. VAT. | 5 ml per Pack Qty.

Art. No. 6472.2

Not available
from 6 Pack Qty. €109.20/Pack Qty.
from 24 Pack Qty. €103.46/Pack Qty.
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Product details


Versatile reagent for gel loading and simultaneous staining of double stranded nucleic acid in agarose- and polyacrylamide gels.

  • Alternative for ethidium bromide, non-toxic, non-mutagen
  • Nearly as sensitive as ethidium bromide, up to 1,5 ng/mm2 nucleic acid
  • For use with common broad-band ethidium bromide photo filters
  • Excitation possible via UV light (320 nm) and blue light (~470 nm)
  • Compatible with all usual down-stream applications

ROTI®Load DNAstain detects up to 0,2 ng per band, and is, therefore, as sensitive as ethidium bromide. Due to its most simple application as gel loading buffer it reduces the time needed for gel analysis significantly. This innovative reagent contains the dye EvaGreen®, which has been developed specifically for DNA analysis. DNA integrity is not influenced by EvaGreen®; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligation, PCR, transformation, transfection etc. without interfering effects. Due to the low membrane permeability of the dye, ROTI®Load DNAstain is non mutagenic, non toxic, and environment friendly.

ROTI®Load DNAstain can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also Rotiphorese® PROfessional runVIEW, Art. No. 4849.1). When excited, nucleic acid-bound ROTI®Load DNAstain emits a brightly coloured green fluorescence (525 nm) that may be documented by all usual broadband ethidium bromide- or SYBR® Green foto filters.


Wavelength

Excitation maximum (bound to DNA): approx. 320 nm and 470-500 nm
Emission maximum (bound to DNA): 525 nm



ROTI®Load DNAstain 3 6x konz., ready-to-use, DNase-free
Directions for use

ROTI®Load DNAstain 3 is a special gel loading buffer with orange G as the only dye optimized for very short runs. Orange G runs in the very small fragment range and shows the maximum running distance for very short runs (see lane 6 in the product image). Also well suited if bromophenol blue or xylene cyanol could mask important DNA bands.

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The figure depicts 50 ng DNA per band, or 75 ng (1000 bp and 2,5 kb), respectively. Each lane has been loaded and concurrently stained using 1 µl Roti®-Load DNAstain.

Glycerin is the standard reagent for increasing sample density.

HP03

Recommended for staining of dsDNA- and dsRNA-fragments of under 500 bp.



Technical Information
Excitation max. (DNA-bound) ca. 320  nm
470–500  nm
Emission max. (DNA-bound) 525 nm  
Dye Orange G, EvaGreen®  
Incl. DNA staining Yes  
Use Simultaneous gel staining, small fragments  
ROTI®Load DNAstain 3
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6472.2 5 ml plastic 5 x 1 ml €114.95
6472.1 1 ml plastic 1 x 1 ml €37.55
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