Technical Data Sheet
SYBR® Green DNA dye, 9.0 ml, 5 x 1.8 ml
Products with optimised manufacturing process in terms of sustainability.
Ready-to-use products and reagents
Boiling point (bp) 100 °C
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
1.8 ml are sufficient for gel run and staining of approx. 3,600 lanes (5 µl sample + 0.5 µl dye).
SYBR® Green I bounds to the minor groove of DNA and intercalates into the DNA. Upon excitation it emits a bright green fluorescence. The sensitivity is up to 0.01 ng per band and is therefore 20 times higher than ethidium bromide.
SYBR® Green I has been developed specifically for gentle DNA analysis. DNA integrity is not influenced; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligations, PCR, transformation, transfection etc. without interfering effects.
€117.20/Pack Qty.
excl. VAT. | 9 ml per Pack Qty.
Art. No. 1CN2.2
Fits to / Accessories
Product details
This is the DNA dye SYBR® Green I, a staining reagent for detection of double stranded nucleic acid in agarose and polyacrylamide gels. The dye is added directly to the DNA/loading buffer mixture and then loaded onto the gel.
SYBR® Green I bounds to the minor groove of DNA and intercalates into the DNA. Upon excitation it emits a bright green fluorescence. The sensitivity is up to 0.01 ng per band and is therefore 20 times higher than ethidium bromide.
SYBR® Green I has been developed specifically for gentle DNA analysis. DNA integrity is not influenced; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligations, PCR, transformation, transfection etc. without interfering effects.
- Recommended for dsDNA
- Direct addition to DNA sample
- For excitation with UV light and blue light
- Non-toxic alternative for ethidium bromide
- Sensitivity (0,01 ng/band)
SYBR® Green DNA dye can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE®PROfessional runVIEW, Art. No. 4849.1). The gels may be documented by all usual broadband ethidium bromide- or SYBR® Green foto filters.
Excitation maximum (bound to DNA): approx. 254 nm and 495 nm
Emission maximum (bound to DNA): 521 nm
SYBR® Green DNA dye 11x conc., for electrophoresis, ready-to-use
| Max. excitation (DNA-bound) |
254 nm 495 nm |
| Amount of traces | ca. 2000/ml |
| Emissions max. (DNA-bound) | 521 nm |
| Sensitivity | 0,01 ng/band |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
|---|---|---|---|---|---|---|
| 1CN2.1 | 1.8 ml | plastic | 1 x 1.8 ml |
€28.95 |
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| 1CN2.2 | 9 ml | plastic | 5 x 1.8 ml |
€117.20 |
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In stock
Available
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
Summary Staining of Nucleic Acid in Gels
| Product | Sensitivity | Description | Excitation |
| Ethidium bromide solution | 0.5-5 ng/band | Standard fluorescent staining | 254-330 nm, 500 nm UV- and blue light |
| SYBR® Green DNA dye | 0.01 ng/band | Non-toxic, green fluorescent staining | 254 nm and 495 nm UV- and blue light |
| ROTI®GelStain | 0.3 ng/band | Non-toxic, green fluorescent staining | 302 nm and 490 nm UV- and blue light |
| ROTI®GelStain Red | 0.3 ng/band | Non-toxic, red fluorescent staining | 310 nm, 540 nm UV- and blue light |
| ROTI®Methylene blue staining concentrate | 10 ng/band | Reversible, non-toxic blue staining | White light |
| ROTI®Black N | <0.1 ng/band | Silver staining of DNA polyacrylic amide gels | White light |
Certificates of Analysis
Type analysis
| Appearance | orange solution |
| Each batch is functionally tested for its suitability in electrophoresis. | |