Technical Data Sheet
ROTI®GelStain Advanced, 5 ml, 5 x 1 ml
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The products marked accordingly are shipped refrigerated in special, sustainable cooling boxes with cooling packs or dry ice.
Ready-to-use products and reagents
Additional transport costs are incurred for this product.
Boiling point (bp) 100 °C
Storage temp. +4 °C
Transport temp. cooled
For non-toxic fluorescence staining of nucleic acids in agarose and polyacrylamide gels. Green fluorescence. Direct application to the agarose solution and the running buffer.
€650.40/Pack Qty.
excl. VAT. | 5 ml per Pack Qty.
Art. No. 2E09.2
Fits to / Accessories
Product details
Non-toxic and non-carcinogenic dyes for fluorescent staining of nucleic acids in agarose gels and polyacrylamide gels. Highly sensitive and applied just like ethidium bromide.
The ROTI®GelStain dyes are used as additives to the gel solution and the running buffer, partly also post-run staining is possible. Both ROTI®GelStain dyes can be excited by UV light and by blue light. Resulting signals may be detected by ethidium bromide broadband photo filters.
ROTI®GelStain Advanced 20 000x conc., ready-to-use
ROTI®GelStain Advanced is a versatile staining reagent for the detection of all nucleic acids in agarose and polyacrylamide gels.
- Simple differentiation between DNA and RNA by characteristic fluorescence: green for DNA and red for RNA
- Alternative to ethidium bromide, non-toxic, non-mutagenic
- Up to 4 x as sensitive as ethidium bromide (approx. 0.1 ng/band)
- Can be used with the usual green filters
- Excitation with UV light (300 nm) and blue light (500 nm) possibleCompatible with all common down-stream applications
ROTI®GelStain Advanced is a green fluorescent dye for nucleid acid staining like ethidium bromide. The nucleic acids are stained during the gel run after adding the dye to the liquid gel and the running buffer.
ROTI®GelStain Advanced has a sensitivity of 0.1 ng/band and is up to 4 x more sensitive than ethidium bromide.
After binding to the nucleic acid in the agarose gel, ROTI®GelStain Advanced emits a bright green fluorescence when bound to DNA and a bright red fluorescence when bound to RNA. The fluorescence can be documented with all common green filters.
ROTI®GelStain Advanced is compatible with all common down-stream applications.
Excitation maximum (bound to DNA): 490 nm
Emission maximum (bound to DNA): 520 nm
Emission maximum (bound to RNA): 635 nm
| Max. excitation (DNA-bound) | 490 nm |
| Emissions max. (DNA-bound) | 520 nm |
| Colour of the solution | green |
| Additional info | Store in the dark at +4°C. Do not freeze! |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
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| 2E09.1 | 1 ml | plastic | 1 x 1 ml |
€134.40 |
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| 2E09.2 | 5 ml | plastic | 5 x 1 ml |
€650.40 |
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In stock
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- Subtotal: 0.00
Downloads / MSDS
General information
Summary Staining of Nucleic Acid in Gels
| Product | Sensitivity | Description | Excitation |
| Ethidium bromide solution | 0.5-5 ng/band | Standard fluorescent staining | 254-330 nm, 500 nm UV- and blue light |
| SYBR® Green DNA dye | 0.01 ng/band | Non-toxic, green fluorescent staining | 254 nm and 495 nm UV- and blue light |
| ROTI®GelStain | 0.3 ng/band | Non-toxic, green fluorescent staining | 302 nm and 490 nm UV- and blue light |
| ROTI®GelStain Red | 0.3 ng/band | Non-toxic, red fluorescent staining | 310 nm, 540 nm UV- and blue light |
| ROTI®Methylene blue staining concentrate | 10 ng/band | Reversible, non-toxic blue staining | White light |
| ROTI®Black N | <0.1 ng/band | Silver staining of DNA polyacrylic amide gels | White light |
Certificates of Analysis
Type analysis
| Appearance | green, transparent solution, no precipitation |
| Staining of DNA in agarose gels | complies |
| Staining of DNA in PA gels | complies |
| DNase, RNase | not detectable |