ROTI®Blue, 250 ml
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
ADR 8 III
1 l concentrated solution is sufficient for approx. 50 mini gels.
excl. VAT. | 250 ml per Pack Qty.
Art. No. A152.2
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ROTI®Blue 5x conc.
ROTI®Blue is a colloidal Coomassie® brilliant blue G250 staining agent for proteins in polyacrylamid gels. Its colloidal features enable the staining agent to be bound to the proteins with high specificity and only minimally to the gel matrix. There is no longer any need for the extensive washing steps for background decoloring.
- Most simple application
- No destaining necessary
- 5x more sensitive than Coomassie® R250
A: Horizontal IEF for determination of wheat-varieties.
With kind permission of R. Knoblauch, LTZ Referat 23, Karlsruhe
B: SDS-PAGE ROTI®Mark Standard.
ROTI®Blue is approximately 5 x more sensitive than standard Coomassie® staining solutions. Staining time takes 2 to 12 hours depending on the desired band intensity.
Due to the fact that proteins may be stained without fixing the gel, membrane transfer and gel elution are still possible following staining.
Particularly well suited for staining prior to MS-analyses (e.g. MALDI) and prior to gel elution.
|Use||Coomassie® staining solution for colloidal staining of proteins. Destaining not required. Non-fixing staining for protein gel elution possible. Proteomics-compatible.|
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Available at short notice
Delivery date currently unknown
|Appearance||dark blue, colloidale solution|
|pH-value||1 - 4|