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ROTIPHORESE®Blue R

ROTIPHORESE® concentrate
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Danger
H225-H301+H311+H331-H315-H319-H370
i highly flammable liquid and vapour, toxic if swallowed, in contact with skin or if inhaled, causes skin irritation, causes serious eye irritation, causes damage to organs (eye)
P210 P280 P302+P352 P304+P340 P305+P351+P338 P308+P311
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Pack Qty.
Pack.
Coomassie®-Staining solution
Density (D) 0,8 g/cm³
Boiling point (bp) >65 °C
Flash point (flp) >10 °C
ADR 3 II
WGK 2
UN-Nr. 3286

Brillant Blue R solution for Coomassie® staining of protein gels.
600 ml concentrated solution is sufficient for approx. 12 mini gels.

Concentrate for easy preparation of Coomassie® Blue R staining solutions.
ROTIPHORESE®Blue R is ideal for staining proteins in SDS-PAGE gels. It saves any tedious weighing of staining powder and having to prepare the mixture.
Product details

€39.25/Pack Qty. 

excl. VAT. | 600 ml per Pack Qty.

Art. No. 3074.1

In stock
Delivery fast, simple and reliable!
from 6 Pack Qty. €37.29/Pack Qty.
from 24 Pack Qty. €35.32/Pack Qty.

Product details



ROTIPHORESE®Blue R ROTIPHORESE® concentrate

Concentrate for easy preparation of Coomassie® Blue R staining solutions.
ROTIPHORESE®Blue R is ideal for staining proteins in SDS-PAGE gels. It saves any tedious weighing of staining powder and having to prepare the mixture.

3074_Bande
Figure:
Gel stained by ROTIPHORESE®Blue R, 12% PAA in Tris, ROTI®Mark STANDARD (Art. No. T851).

Directions for use

Concentrate contains Coomassie® Blue R, methanol and pure acetic acid. ROTIPHORESE®Blue R can be diluted directly in the bottle with 400 to 600 ml deionized water, resulting in the following composition depending on the dilution.

When diluted with 397,5 ml water (to a total of 1 litre staining solution): Methanol (50 %), acetic acid 10 %, Coomassie® R (0,25 %)
When diluted with 507,5 ml water (to a total of 1,1 litre staining solution): Methanol (45 %), acetic acid 9 %, Coomassie® R (0,22 %)
When diluted with 602,5 ml water (to a total of 1,2 litre staining solution): Methanol (41,5 %), acetic acid 8,3 %, Coomassie® R (0,2 %).


We recommend mixing the concentrated solution well before dilution and filtering the diluted solution before application.



Technical Information
Sensitivity 50 
Use Coomassie® staining solution concentrate for rapid staining 
ROTIPHORESE®Blue R
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3074.1 600 ml glass

€39.25

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Downloads / MSDS


General information

Suitability of Protein Stainings

Product Sensitivity Rapid Gel

Analysis
Gel

Drying
Elution/

Transfer
2D-

Gels
MS-

Analysis
ROTI®Black P 1 ng/mm2 - +++ - +++ -
ROTI®Blue 10 ng/mm2 - +++ +++ - +++
ROTI®Blue quick 10 ng/mm2 +++ +++ ++ - ++
ROTI®Blau R 50 ng/mm2 +++ + - - -
+++: recommended +: suitable -: not recommended

Summary Staining of Protein Gels

Product Sensitivity Description
ROTI®Black P 1 ng/mm2 Silver staining kit. Highly sensitive. Simple, fast and reproducible.
ROTI®Blue 10 ng/mm2 Coomassie®-solution for colloidal staining of proteins. Destaining not required. Non-fixing staining for protein gel elution possible. Proteomics-compatible.
ROTI®Blue quick 10 ng/mm2 Solution for rapid, highly specific staining of proteins. Destaining not required. Non-fixing staining, compatible with blotting and Proteomics.
ROTIPHORESE®Blau R 50 ng/mm2 Coomassie® staining solution concentrate for rapid staining.
Coomassie® Blue G250 (C.I. 42655) 60 ng/mm2 Dye for performing Coomassie® staining. ODmax = 595
Coomassie® Blue R250 (C.I. 42660) 50 ng/mm2 Dye for performing Coomassie staining. ODmax = 550

Certificates of Analysis

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