Jump to content Jump to navigation menu

4-Nitrophenyl phosphate disodium salt hexahydrate, 25 g

≥99 %, p.a.
Pack Qty.
Pack.
pNPP, p-Nitrophenyl phosphate
Empirical formula C6H4NNa2O6P · 6 H2O
Molar mass (M) 371,12 g/mol
Melting point (mp) >300 °C
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
CAS No. [4264-83-9]
EG-Nr. 224-246-5

Substrate for alkaline phosphatase, particularly for ELISA.
Application: Working solution: 1 mg/ml in diethanolamine substrate buffer (10 mM diethanolamine, 0.5 mM MgCl2, pH 9.5). For endpoint analysis the reaction may be stopped via addition of 2-3 M NaOH.

Substrate of the alkaline phosphatase for use in ELISAs. During reaction of the substrate, a soluble, yellow product is formed that is detected photometrically at 405 nm wave length.
Product details

€69.80/Pack Qty. 

excl. VAT. | 25 g per Pack Qty.

Art. No. 4165.9

Available at short notice
from 6 Pack Qty. €66.31/Pack Qty.
from 24 Pack Qty. €62.82/Pack Qty.
Carl Roth subscription service

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:
1

Put all products for your subscription in the desired quantity in the basket.

2

In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.

3

Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Product details



4-Nitrophenyl phosphate disodium salt hexahydrate ≥99 %, p.a.

Substrate of the alkaline phosphatase for use in ELISAs. During reaction of the substrate, a soluble, yellow product is formed that is detected photometrically at 405 nm wave length.



Technical Information
Enzyme Alkaline phosphatase 
Detection Colour (yellow) 
Signal product soluble (ELISA, activity assay) 
4-Nitrophenyl phosphate disodium salt hexahydrate
Selected quantity:   0
  1. Subtotal:  0.00
Order No. Pack Qty. Pack. Price Quantity
4165.1 2.5 g glass €17.10
4165.7 100 g glass €188.15
4165.9 25 g glass €69.80
In stock
Available at short notice
Not available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS


General information

Assay protocol for use of BCIP/NBT in immunoblot procedures:

Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.


Detection of peroxidase activity (Immunoassay):

Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).

Always prepare freshly!

Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.

Reference: Bos, E.S. et al., (1981) J. Immunoassay 2, (3/4), 187.


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Guarantee analysis

Assay (HPLC)≥99,0 %
Solution (5 % in H2O)clear, colourless to yellow
Free 4-nitrophenol≤0,01 %

0 Reviews

0.0 out of five stars