Technical Data Sheet
X-α-Gal, 25 mg
Molar mass (M) 405,63 g/mol
Density (D) 1,882 g/cm³
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
CAS No. 107021-38-5
Colorimetric substrate of α-galactosidase.
Used for selection of positive clones in yeast two-hybrid screens.
€88.05/Pack Qty.
excl. VAT. | 25 mg per Pack Qty.
Art. No. HN36.1
Product details
X-α-Gal ≥98 %, for biochemistry
Chromogenic substrate of α-galactosidase (melibiase, α-D-galactoside galactohydrolase), an enzyme that is necessary for metabolization of the disaccharide melibiose as a carbon source.
Used for selection of positive clones in yeast two-hybrid screens.
Working concentration: 20-50 µg/ml in agar plates.
| Enzyme | α-D-Galactosidase |
| Detection | Colour (blue) |
| Signal product | Precipitate (Yeast-Two-Hybrid) |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| HN36.1 | 25 mg | glass |
€88.05 |
|
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| HN36.2 | 100 mg | glass |
€263.40 |
|
|
| HN36.3 | 250 mg | glass |
€622.45 |
|
|
|
In stock
Available
In procurement
No longer available
Delivery date currently unknown
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- Subtotal: 0.00
Downloads / MSDS
General information
Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.
Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).
Always prepare freshly!
Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.
Reference: Bos E.S. et al. (1981) J. Immunoassay 2, (3/4), 187.
Certificates of Analysis
Type analysis
| Assay | ≥98 % |
| Water (KF) | ≤1.0 % |
| Specific rotation [α]20D (c=1 in DMF/water 1:1) | +140° to +150° |