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X-Gluc, 100 mg

≥99 %, for microbiology
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X-GlcA, 5-Bromo-4-chloro-3-indolyl-β-D-glucuronide monohexyl ammonium salt
Empirical formula C20H26N2O7ClBr · H2O
Molar mass (M) 539,81 g/mol
Melting point (mp) 230 °C
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
CAS No. [114162-64-0]

Glucuronidase substrate

For colorimetric detection of glucuronidase activity. During hydrolysis of X-gluc two molecules are formed, glucuronic acid and 5-bromo-4-chloro-indoxyle, which is then being oxidized to a deep-blue indigo-dye.
Product details

€110.20/Pack Qty. 

excl. VAT. | 100 mg per Pack Qty.

Art. No. 0018.1

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Product details



X-Gluc ≥99 %, for microbiology

For colorimetric detection of glucuronidase activity. During hydrolysis of X-gluc two molecules are formed, glucuronic acid and 5-bromo-4-chloro-indoxyle, which is then being oxidized to a deep-blue indigo-dye.


X-Gluc is used for detection of contaminations by E. coli bacteria in food, waters and feeds. In molecular genetics, it is widely used as marker for expression analysis of target genes.



Technical Information
Enzyme β-D-Glucuronidase 
Detection Colour (blue) 
Signal product Precipitate (detection of living cells) 
X-Gluc
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0018.1 100 mg glass €110.20
0018.2 500 mg glass €413.90
0018.3 1 g glass €686.95
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Downloads / MSDS


General information

Assay protocol for use of BCIP/NBT in immunoblot procedures:

Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.


Detection of peroxidase activity (Immunoassay):

Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).

Always prepare freshly!

Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.

Reference: Bos, E.S. et al., (1981) J. Immunoassay 2, (3/4), 187.


Certificates of Analysis

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Guarantee analysis

Appearancewhite to off-white powder
Purity (HPLC)≥99.0 %
Solution (1% in DMF/Wasser 1:1)clear, colorless
Water (KF)≤5.0 %
Specific rotation [α]a (c=1 in DMF/water 1:1)-91.0° to -87.0°

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