Jump to content Jump to navigation menu

3,3',5,5'-Tetramethylbenzidine, 5 g, plastic

≥98 %, p.a.
Pack Qty.
Pack.
TMB
Empirical formula C16H20N2
Molar mass (M) 240,35 g/mol
Density (D) 0,45 g/cm³
Melting point (mp) 170 °C
Storage temp. +4 °C
WGK 1
CAS No. [54827-17-7]
EG-Nr. 259-364-6

Substrate for peroxidase.
Working solution: 1 mg TMB in 0.1 ml dimethyl sulfoxide, add 9.9 ml 0.1 M sodium acetate (pH 6). Filter and add H2O2 ad 0.01 %. Always prepare freshly!

Application: approx. 50 µl per 96well, incubate for 10-30 mins. at room temperature. Add 50 µl 1 M H2SO4 and quantitate photometrically at 450 nm (acc. to: Bos et. al. (1981) J. Immunoassay 2:187).

Protect from light.

Used as a highly sensitive substrate for peroxidases, particularly in ELISA procedures. Can replace carcinogenic benzidine.

Dissolves easily in acetone, chloroform and ethanol.
Product details

€170.95/Pack Qty. 

excl. VAT. | 5 g per Pack Qty.

Art. No. 6350.2

In stock
Delivery fast, simple and reliable!
from 6 Pack Qty. €162.40/Pack Qty.
from 24 Pack Qty. €153.86/Pack Qty.
Carl Roth subscription service

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:
1

Put all products for your subscription in the desired quantity in the basket.

2

In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.

3

Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Product details



3,3',5,5'-Tetramethylbenzidine ≥98 %, p.a.

Used as a highly sensitive substrate for peroxidases, particularly in ELISA procedures. Can replace carcinogenic benzidine.

Dissolves easily in acetone, chloroform and ethanol.



Technical Information
Enzyme Horseradish Peroxidase 
Detection Colour (blue) 
Signal product soluble (ELISA) 
3,3',5,5'-Tetramethylbenzidine
Selected quantity:   0
  1. Subtotal:  0.00
Order No. Pack Qty. Pack. Price Quantity
6350.1 1 g glass €56.90
6350.2 5 g plastic €170.95
6350.3 25 g plastic €568.70
In stock
Available at short notice
Not available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS


General information

Assay protocol for use of BCIP/NBT in immunoblot procedures:

Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.


Detection of peroxidase activity (Immunoassay):

Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).

Always prepare freshly!

Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.

Reference: Bos, E.S. et al., (1981) J. Immunoassay 2, (3/4), 187.


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Appearancebeige cryst. powder
Assay (GC)≥99.0 %
Sulphated ash≤0,2 %
Loss on drying (3 h, 60 °C, vacuum)≤1,0 %
Melting range166-171 °C
Can replace carcinogenic benzidine.

0 Reviews

0.0 out of five stars