3,3',5,5'-Tetramethylbenzidine, 25 g, plastic
Molar mass (M) 240,35 g/mol
Melting point (mp) 170 °C
Storage temp. +4 °C
WGK 1
CAS No. 54827-17-7
EG-Nr. 259-364-6
Always prepare a fresh working solution!
€639.65/Pack Qty.
excl. VAT. | 25 g per Pack Qty.
Art. No. 6350.3
Now recurring orders conveniently delivered as a subscription!
With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!
How it works:Put all products for your subscription in the desired quantity in the basket.
In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.
Set Starty point and interval for your subscription and submit order!
By the way: Through your account you can customize or delete your subscriptions anytime.
Product details
3,3',5,5'-Tetramethylbenzidine ≥98 %, p.a.
Used as a highly sensitive substrate for peroxidases, particularly in ELISA procedures. Can replace carcinogenic benzidine.
Dissolves easily in acetone, chloroform and ethanol.
Working solution: 1 mg TMB in 0.1 ml dimethyl sulfoxide, add 9.9 ml 0.1 M sodium acetate (pH 6). Filter and add H2O2 ad 0.01 %. Always prepare freshly!
Application: approx. 50 µl per 96well, incubate for 10-30 mins. at room temperature. Add 50 µl 1 M H2SO4 and quantitate photometrically at 450 nm (acc. to: Bos et. al. (1981) J. Immunoassay 2:187).
Protect from light.
| Enzyme | Horseradish Peroxidase |
| Detection | Colour (blue) |
| Signal product | soluble (ELISA) |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | ||
|---|---|---|---|---|---|---|
| 6350.1 | 1 g | glass |
€62.25 |
|
||
| 6350.2 | 5 g | plastic |
€192.45 |
|
||
| 6350.3 | 25 g | plastic |
€639.65 |
|
||
|
In stock
In procurement
Not available
Delivery date currently unknown
|
||||||
- Subtotal: 0.00
Downloads / MSDS
General information
Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.
Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).
Always prepare freshly!
Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.
Reference: Bos E.S. et al. (1981) J. Immunoassay 2, (3/4), 187.
Certificates of Analysis
Type analysis
| Appearance | beige cryst. powder |
| Assay (GC) | ≥99.0 % |
| Sulphated ash | ≤0,2 % |
| Loss on drying (3 h, 60 °C, vacuum) | ≤1,0 % |
| Melting range | 166-171 °C |
| Can replace carcinogenic benzidine. | |