Jump to content Jump to navigation menu

5-Bromo-4-chloro-3-indolyl phosphate disodium salt, 5 g

≥99 %
Pack Qty.
BCIP-Na2, X-Phosphate disodium salt, X-Phos disodium salt
Empirical formula C8H4BrClNO4PNa2 · 1,5 H2O
Molar mass (M) 397,46 g/mol
Storage temp. -20 °C
Transport temp. ambient temp.
CAS No. [102185-33-1]

Substrate for alkaline phosphatase, in particular in immunoblot procedures.
Highly soluble in water (20 mg/ml), poorly soluble in DMF.

Protect from light and humidity.

In combination with NBT ideal for colorimetric detection of alkaline phosphatase activity, in particular in immunoblot procedures.
Product details

€697.70/Pack Qty. 

excl. VAT. | 5 g per Pack Qty.

Art. No. A155.4

In stock
Delivery fast, simple and reliable!
from 6 Pack Qty. €662.82/Pack Qty.
from 24 Pack Qty. €627.93/Pack Qty.
Carl Roth subscription service

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:

Put all products for your subscription in the desired quantity in the basket.


In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.


Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Product details

5-Bromo-4-chloro-3-indolyl phosphate disodium salt ≥99 %

In combination with NBT ideal for colorimetric detection of alkaline phosphatase activity, in particular in immunoblot procedures.

Technical Information
Application Western, in situ, Histochemistry 
Enzyme Alkaline phosphatase 
Detection Colour (brown-violet) 
Signal product Precipitate (Blot) 
Use Western, in situ, histochimie 
5-Bromo-4-chloro-3-indolyl phosphate disodium salt
Selected quantity:   0
  1. Subtotal:  0.00
Order No. Pack Qty. Pack. Price Quantity
A155.1 100 mg glass €30.00
A155.2 500 mg glass €126.35
A155.3 1 g glass €213.95
A155.4 5 g glass €697.70
In stock
Available at short notice
Not available
Delivery date currently unknown
Selected quantity:   0
  1. Subtotal:  0.00

Downloads / MSDS

General information

Assay protocol for use of BCIP/NBT in immunoblot procedures:

Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.

Detection of peroxidase activity (Immunoassay):

Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).

Always prepare freshly!

Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.

Reference: Bos, E.S. et al., (1981) J. Immunoassay 2, (3/4), 187.

Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Appearancealmost white powder
Assay (titr.)≥99,0 %
Water (KF)5,8-7,8 %

0 Reviews

0.0 out of five stars