p-Nitrotetrazolium blue chloride, 1 g
Molar mass (M) 817,65 g/mol
Melting point (mp) 189 °C
Storage temp. +4 °C
CAS No. [298-83-9]
excl. VAT. | 1 g per Pack Qty.
Art. No. 4421.3
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p-Nitrotetrazolium blue chloride ≥98 %, p.a.
Used in combination with BCIP-toluidine salt (X-phosphate) for detection of alkaline phosphatase activity by producing an indigo dye precipitate. Colour enhancer for staining with alkalic phosphatase. During dimerisation of ketonized X-Phos, H+ ions are set free which reduce NBT to purple-coloured diformazan. Both dyes precipitate in the direct vicinity of the phosphatase, hence staining this site in a dark violet. Store at +4 °C. Please also see assay protocol for use of BCIP/NBT in immunoblot procedures.
|Use||Western, in situ, Histochemistry|
|Signal product||Präzipitat (Blot)|
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|Assay (NT)||≥98 %|
|Water (KF)||≤5,0 %|