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p-Nitrotetrazolium blue chloride, 1 g

≥98 %, p.a.
Pack Qty.
Nitro-BT, NBT, Nitroblue tetrazolium chloride
Empirical formula C40H30Cl2N10O6
Molar mass (M) 817,65 g/mol
Melting point (mp) 189 °C
Storage temp. +4 °C
CAS No. [298-83-9]
EG-Nr. 206-067-4

Application: Stock solution: 0.5 g NBT in 10 ml 70 % dimethylformamide. Protect from light and humidity.

Used in combination with BCIP-toluidine salt (X-phosphate) for detection of alkaline phosphatase act ...
Product details
Type analysis

€84.85/Pack Qty. 

excl. VAT. | 1 g per Pack Qty.

Art. No. 4421.3

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Product details

p-Nitrotetrazolium blue chloride ≥98 %, p.a.

Used in combination with BCIP-toluidine salt (X-phosphate) for detection of alkaline phosphatase activity by producing an indigo dye precipitate. Colour enhancer for staining with alkalic phosphatase. During dimerisation of ketonized X-Phos, H+ ions are set free which reduce NBT to purple-coloured diformazan. Both dyes precipitate in the direct vicinity of the phosphatase, hence staining this site in a dark violet. Store at +4 °C. Please also see assay protocol for use of BCIP/NBT in immunoblot procedures.

Technical Information
Use Western, in situ, Histochemistry  
Enzyme Alkalische Phosphatase  
Detection Farbe (braun-violett)  
Signal product Präzipitat (Blot)  
p-Nitrotetrazolium blue chloride
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Order No. Pack Qty. Pack. Price Quantity
4421.1 100 mg glass €17.75
4421.2 500 mg glass €44.65
4421.3 1 g glass €84.85
4421.4 5 g glass €306.40
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Analysis certificates

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Type analysis

Assay (NT)≥98 %
Water (KF)≤5,0 %

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