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ROTI®Lumin, 2 x 120 ml

10x conc., fluorescence-free, for protein and cytoimmunochemistry
Icon_ready-to-use Ready-to-use products and reagents
Pack Qty.
HRP substrate
Storage temp. +4 °C

Chemoluminescence substrate for peroxidase mediated detection systems.
2 x 120 ml is sufficient for approx. 3,500 cm2 membrane (approx. 50 mini gel blots) or 2,400 wells in the ELISA.

€482.70/Pack Qty. 

excl. VAT. | 1 kit per Pack Qty.

Art. No. P078.1

In production
from 6 Pack Qty. €458.56/Pack Qty.
from 24 Pack Qty. €434.43/Pack Qty.

Product details

ROTI®Lumin 10x conc., fluorescence-free, for protein and cytoimmunochemistry

Technical Information
Application Western, in situ, Histochemistry 
Enzyme Horseradish Peroxidase 
Detection chemoluminescent 
Signal product Precipitate (Blot) 
Use Western 
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Art. No. Pack Qty. Pack. Packaging Price Quantity
P078.1 1 kit plastic 2 x 120 ml


P078.2 1 kit plastic 2 x 25 ml


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Downloads / MSDS

General information

Assay protocol for use of BCIP/NBT in immunoblot procedures:

Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.

Detection of peroxidase activity (Immunoassay):

Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).

Always prepare freshly!

Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.

Reference: Bos E.S. et al. (1981) J. Immunoassay 2, (3/4), 187.

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