Trypsin inhibitor, 1 g
CAS No. [9035-81-8]
Stock solution: 1-10 mg/ml in H2O, PBS or other phosphate buffer.
Recommended final concentration: 1-100 µg/ml.
Storage of stock solution: in aliquots at -20 °C.
excl. VAT. | 1 g per Pack Qty.
Art. No. 2949.5
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Trypsin inhibitor ≥10 000 BAEE U/mg
Trypsin inhibitor inhibits Trypsin with high effiency, and, to a lesser extent, chymotrypsin. Additionally, there is a light inhibiting effect on plasmin, kallikrein, thrombin and a few other proteolytic enzymes.
1 mg of lyophilisate inhibits at minimum 10.000 BAEE Units of trypsin.
Application in cell culture (stock solution 1 mg/ml): Following trypsination, add the same volume inhibitor solution as was used for trypsin. Zentrifuge cells (5 mins, 1000 rpm), and remove supernatant. Resuspend cells in medium and cultivate further.
Application: Solutions of higher concentration may be hazy and slightly yellow-coloured.
pH optimum: pH 7.6-8.0.
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|Order No.||Pack Qty.||Pack.||Price||Quantity|
Available at short notice
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|Colour||beige to light yellow|
|Dry extract||>930 mg/g|
|pH-value (1 % solution)||7,0 ±0,8|
|Activity||≥10000 BAEE U/mg|