ROTITEST®Vital, 5 ml, 1 x 5 ml
Stabile at ambient temperature. Very stabile when being cooled.
Store protected from light. Do not freeze.
1 ml is sufficient for enumeration of 100 cell samples.
excl. VAT. | 5 ml per Pack Qty.
Art. No. 0069.1
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Rapid and simple test system for non-radioactive quantitation of proliferating cells. Also well suitable for cytotoxicity assays.
- Cells keep on being vital
- Most simple handling
- One-component-system without radioactivity
- For adherent and suspension cells
- Photometric result in 1 to 4 hours
- Strong correlation between absorbance and cell number
- Suitable for every culture media
ROTITEST®Vital ROTITEST® Bio Analysis
Sensitivity of the tetrazolium salt used is higher than that known for standard reagents such as MTT, XTT, MTS, or WST-1. Results were shown to be of highly stringent correlation with [3H]-thymidine incorporation assays, therefore representing viability as well as proliferation status of the cells (Tominaga et al., Anal. Commun. 1999 (36) 47-50).
In all living cells, NADH and NADPH are formed by the respiratory chain. ROTITEST®vital is based on the (colourless) WST-8, which functions as acceptor for the NADH/NADPH dehydrogenase while being reduced to (coloured) formazane during the process.
The test solution is added directly to the cultivated cells in 96wells. During incubation for few hours, a water-soluble formazan dye is produced by viable cells, and is released into the surrounding medium. The resulting orange colour is measured photometrically (approx. 450 nm).
Also suitable for culture media containing phenol red.
Each package contains simple-to-follow instructions-for-use.
Fig. D: Proliferation assay on CTLL-2 cells (in RPMI) under influence of humane IL-2 (exposition 72 h), using ROTITEST® Vital (10 μl/well).
- Subtotal: 0.00
|Order No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|0069.1||5 ml||plastic||1 x 5 ml||€177.40||
|0069.2||20 ml||plastic||4 x 5 ml||€532.15||
|0069.3||1 ml||plastic||1 x 1 ml||€63.35||
Available at short notice
Delivery time on request
|Appearance (test solution)|