ROTITEST®Vital, 1 ml, 1 x 1 ml

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Technical Data Sheet

0069_RotitestVital-Substanztest-Ablaufschema

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Type analysis

ROTITEST^®Vital, 1 ml, 1 x 1 ml

ROTITEST^® BioAnalysis Grade, sterile, ready-to-use

Downloads / MSDS

Certificates of Analysis

Type analysis

Sterile products

Ready-to-use products and reagents

Sterile

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Cell proliferation test solution

Density (D) 1,37 g/cm³
Boiling point (bp) 100 °C
Storage temp. +4 °C
WGK 1

Colorimetric test solution for evaluation of cell proliferation and -viability. Non toxic.
Stable at ambient temperature. Very stabile when being cooled.
Store protected from light. Do not freeze.

1 ml is sufficient for 100 measurements.

Rapid and simple test system for non-radioactive quantitation of proliferating cells. Also well suitable for cytotoxicity assays.

Product details

€80.55/Pack Qty.

excl. VAT. | 1 ml per Pack Qty.

Art. No. 0069.3

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from 6 Pack Qty. €76.52/Pack Qty.

from 24 Pack Qty. €72.50/Pack Qty.

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Product details

Sterile

Rapid and simple test system for non-radioactive quantitation of proliferating cells. Also well suitable for cytotoxicity assays.

Optimum replacement for MTT:

Cells keep on being vital
Most simple handling
One-component-system without radioactivity
For adherent and suspension cells
Photometric result in 1 to 4 hours
Strong correlation between absorbance and cell number
Suitable for every culture media

ROTITEST^®Vital ROTITEST^® BioAnalysis Grade, sterile, ready-to-use

Mechanism

Sensitivity of the tetrazolium salt used is higher than that known for standard reagents such as MTT, XTT, MTS, or WST-1. Results were shown to be of highly stringent correlation with [³H]-thymidine incorporation assays, therefore representing viability as well as proliferation status of the cells (Tominaga et al., Anal. Commun. 1999 (36) 47-50).
In all living cells, NADH and NADPH are formed by the respiratory chain. ROTITEST^®vital is based on the (colourless) WST-8, which functions as acceptor for the NADH/NADPH dehydrogenase while being reduced to (coloured) formazane during the process.

Directions for use

The test solution is added directly to the cultivated cells in 96wells. During incubation for few hours, a water-soluble formazan dye is produced by viable cells, and is released into the surrounding medium. The resulting orange colour is measured photometrically (approx. 450 nm).
Also suitable for culture media containing phenol red.

Each package contains simple-to-follow instructions-for-use.

Fig.: Example of use

Fig. A: Correlation of cell counting results achieved with ROTITEST^® Vital (10 μl/well) and ³H-Thymidine (37 KBq/well). HeLa (1, in DMEM) and HL60 cells (2, in MEM).

Fig. B: Comparison of cell counting results using ROTITEST^® Vital (10 μl/well) and other cell counting reagents. Photometrical measurement at 450 nm (ROTITEST^® Vital, XTT), 490 nm (MTS), and 570 nm (MTT). HeLa (1, in DMEM) and HL60 cells (2, in MEM).

Fig. C: Measurement of cytotoxic effects of actinomycin D (exposition 24 h) on HeLa cells (in DMEM), assayed with ROTITEST^® Vital (10 μl/well).

Fig. D: Proliferation assay on CTLL-2 cells (in RPMI) under influence of humane IL-2 (exposition 72 h), using ROTITEST^® Vital (10 μl/well).

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	Art. No.	Pack Qty.	Pack.	Packaging	Price
	0069.1	5 ml	plastic	1 x 5 ml	€224.70 €224.70
	0069.2	20 ml	plastic	4 x 5 ml	€671.90 €671.90
	0069.3	1 ml	plastic	1 x 1 ml	€80.55 €80.55
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