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ROTI®Transform

sterile, ready-to-use
Icon_Steril Sterile products
Icon_ready-to-use Ready-to-use products and reagents
Pack Qty.
Pack.
Packaging
Transformation kit
Storage temp. –20 °C
Transport temp. ambient temp.

For preparation of competent E. coli.
One kit is sufficient to produce competent E. coli for 500 transformations.
Contents of this Kit may not be bought separately.

ROTI®Transform is a simple, effective and fast method of producing competent E. coli in three steps. The competent cells may be used directly for transformation or may be frozen for later transformations.
Product details
€60.90 /Pack Qty.

€47.50/Pack Qty.  Campaign price!

excl. VAT. | 1 kit per Pack Qty.

Art. No. P043.1

Available at short notice
Free of shipping costs from 125 €
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Personal technical advice on this product
Ms Hübner
Ms Hübner
+49 721 5606 - 1034

Product details


ROTI®Transform is a simple, effective and fast method of producing competent E. coli in three steps. The competent cells may be used directly for transformation or may be frozen for later transformations.

  • Flexible selection of strains
  • Effective, simple and fast
  • Adequate for 500 transformations

Transformation efficiency is 5 x 105 to 1 x 106 colonies per µg DNA.



ROTI®Transform sterile, ready-to-use
Directions for use

Protocol:
1. Resuspend cells in fresh, cold LB-medium
2. Add ROTI®Transform 1
3. Add ROTI®Transform 2 and incubate for 5 minutes on ice.


The kit contains

ROTI®Transform consists of 45 ml ROTI®Transform 1 (Art. No. P041) and 5 ml ROTI®Transform 2 (Art. No. P042).

P043_Ablaufschema

User information

Long-term Storage of the Kit at -20 °C.

Storage for use:
Reagent 1: Short-term storage may be done at 4 °C.For long-term storage of over 14 days we recommend a storage temperature of -20 °C.
Reagent 2: Storage at 4 °C.



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P043.1 1 kit cardboard for approx. 500 transformations €47.50
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