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ROTI®Garose-Glu/GST Beads, 10 ml

ROTI®Garose for biochemistry
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H226-H319
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P305+P351+P338
i keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking, wear protective gloves/eye protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Agarose beads for affinity chromatography, Glutathione-agarose beads, Column matrix for protein isolation
Flash point (flp) 35 °C
Melting point (mp) -114 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For isolation of GST-tagged proteins by affinity chromatography
Suitable for batch mode, LPLC, FPLC and MPLC and big matrix volumes

Glutathione coupled agarose beads for affinity chromatography. 75 % bead suspension in 20  ...
Product details
Type analysis

€279.00/Pack Qty. 

excl. VAT. | 10 ml per Pack Qty.

Art. No. 0841.1

In stock
Free of shipping costs from 125 €
Due to the high demand, there may be a delay in shipping of up to 5 working days.
from 6 Pack Qty. €265.05/Pack Qty.
from 24 Pack Qty. €251.10/Pack Qty.
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Product details



ROTI®Garose-Glu/GST Beads ROTI®Garose for biochemistry

Glutathione coupled agarose beads for affinity chromatography. 75 % bead suspension in 20 % ethanol.

ROTI®Garose-Glu/GST Beads offer the perfect solution for your specific application of low flow column chromatography or protein isolation in batch mode, under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.

  • One-step isolation of GST-tagged proteins from total lysates
  • Rapid, mild and highly specific purification
  • Suitable for isolation of GST-peptides and protein complexes of all sizes
  • High binding capacity
  • Easy elution and regeneration
  • For batch mode, gravity flow, MPLC und FPLC
  • Suitable for big matrix volumes

Suitable for

Recommended for isolation of glutathione-S-transferase (GST) or GST fusion proteins in batch mode or via columns. Suitable for low to medium pressure, FPLC, or if GST-tag proteins shall be isolated either in particularly short time or in big amounts.


Directions for use

The matrix of ROTI®Garose-Glu/GST Beads consists of beaded 4 % agarose without crosslinking, coupled with glutathione. The matrix is easy to pack and results in very evenly packed columns. Elution is carried out via a mild Tris/glutathione buffer, enabling even recovery of native protein complexes.
Efficient and highly specific isolation of GST-tagged proteins via affinity chromatography is based on the affinity of the glutathion-S-transferase to it’s substrate glutathione. Merely no unspecific binding of proteins without GST-tag takes place, even during purification from raw extracts, while the biological activity of the purified proteins is preserved by chromatography conditions.
ROTI®Garose-Glu/GST Beads may be regenerated, making them very cost-effective.



ROTI®Garose-Glu/GST Beads
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0841.1 10 ml plastic €279.00
0841.2 100 ml plastic €1,819.00
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Downloads


Analysis certificates

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Particle size
Beads (un-cross-linked agarose)4 %  
LigandGlutathione  
Binding capacity (gel) (GSTrek)≥8 mg/ml  
Ethanol20 %  

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