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ROTI®Garose-Glu/GST Cartridges, 5 ml, 1 x 5 ml

ROTI®Garose for biochemistry
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Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H226-H319
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P305+P351+P338
i keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking, wear protective gloves/eye protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Packaging
Glutathione-agarose beads, Affinity chromatography colums, Column matrix for protein isolation
Flash point (flp) 35 °C
Melting point (mp) -114 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For isolation of GST-tagged proteins by high pressure affinity chromatography
Suitable for MPLC, FPLC, ÄKTA™ and peristaltic pumps

Glutathione coupled agarose beads for medium pressure affinity chromatography (MPLC). Prepacked matrix, stabilised with 20 % ethanol.

ROTI®Garose-Glu/GST Cartridges offer the perfect solution for your specific application of chromatography under medium pressure (MPLC, FPLC).
Product details
Type analysis

€249.00/Pack Qty. 

excl. VAT. | 5 ml per Pack Qty.

Art. No. 0843.1

Available at short notice
Free of shipping costs from 125 €
from 6 Pack Qty. €236.55/Pack Qty.
from 24 Pack Qty. €224.10/Pack Qty.
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Personal technical advice on this product
Ms Hübner
Ms Hübner
+49 721 5606 - 1034

Product details



ROTI®Garose-Glu/GST Cartridges ROTI®Garose for biochemistry

Glutathione coupled agarose beads for medium pressure affinity chromatography (MPLC). Prepacked matrix, stabilised with 20 % ethanol.

ROTI®Garose-Glu/GST Cartridges offer the perfect solution for your specific application of chromatography under medium pressure (MPLC, FPLC).

  • Easy isolation of GST-tagged proteins from total lysates
  • Rapid, mild and highly specific purification
  • Suitable for isolation of GST-peptides and protein complexes of all sizes
  • High binding capacity
  • Easy elution and regeneration
  • Recommended flow rate 0,5-5 ml/min.
  • Standard ports (10-32)
  • For medium pressure flow chromatography
  • Very versatile. Applicable with ÄKTA™FPLC™, pump and syringe

Suitable for

Optimal for isolation of glutathione-S-transferase (GST) or GST fusion proteins via automated liquid chromatography, or if proteins shall be isolated under pressure. Suitable for ÄKTA™FPLC™ and for sample application via peristaltic pump or syringe.


Directions for use

ROTI®Garose-Glu/GST Cartridges are prepacked and may be used directly. The matrix of ROTI®Garose-Glu/GST Cartridges consists of beaded 4 % agarose without crosslinking, coupled with glutathione. Elution is carried out via a mild Tris/glutathione buffer, enabling even recovery of native protein complexes.
Efficient and highly specific isolation of GST-tagged proteins via affinity chromatography is based on the affinity of the glutathion-S-transferase to it’s substrate glutathione. Merely no unspecific binding of proteins without GST-tag takes place, even during purification from raw extracts, while the biological activity of the purified proteins is preserved by chroatography conditions.
ROTI®Garose-Glu/GST Cartridges may be regenerated, making them very cost-effective.
Column material made from polypropylene and polyethylene (frit).



ROTI®Garose-Glu/GST Cartridges
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Order No. Pack Qty. Pack. Packaging Price Quantity
0843.1 5 ml plastic 1 x 5 ml €249.00
0843.2 25 ml plastic 5 × 5 ml €1,029.00
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Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Column volume5 ml  
Particle size50-150
Beads (un-cross-linked agarose)4 %  
LigandGlutathione  
Binding capacity/cartridge (GSTrek)≥50 mg  

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