ROTI®Garose-His/Ni Columns, 8 unit(s)
Ready-to-use products and reagents
Storage temp. +4 °C
ADR 3 II
WGK 3
UN-Nr. 1170
Prepacked colums for gravity flow chromatography.
Matrix volume 1 or 5 ml, whole volume 12 or 35 ml, respectively.
For approx. 100 and 500 mg His labelled protein.
Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort. ROTI®Garose His/Ni Columns offer the perfect solution for fast and simple isolation of His-tagged proteins from total lysates.
€243.90/Pack Qty.
excl. VAT. | 8 unit(s) per Pack Qty.
Art. No. 1314.1
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Product details
ROTI®Garose-His/Ni Columns ROTI®Garose for biochemistry
Prepacked columns with nickel-loaded IDA agarose bead matrix for low-pressure affinity chromatography.
Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort. ROTI®Garose His/Ni Columns offer the perfect solution for fast and simple isolation of His-tagged proteins from total lysates.
- Fast and simple purification of His-tagged proteins from total lysates
- Superior recovery rate of pure His-tagged proteins
- Binding capacity (purified 6xHis protein) of 117 mg per ml matrix
- Easy elution and regeneration
- Very low Nickel leaching
- Compatible with denaturing reagents
- For gravity flow chromatography
Suitable for all general applications, or if proteins of very small amount shall be isolated without loss.
ROTI®Garose His/Ni Columns are prepacked and may be used directly. The matrix consists of cross-linked and beaded 6 % agarose, IDA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two exposed histidines with good specificity and very high affinity, making the Ni2+ charged matrix the first choice for all standard applications. ROTI®Garose His/Ni Columns give very high yields of pure His-tagged protein with considerably low metal contamination. The tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI®Garose Columns may repeatedly be regenerated, making them very cost-effective.
The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT). Suspension in 20 % ethanol.
Column material made from polypropylene and polyethylene (frit).
May be autoclaved at 121 °C for 30 mins.
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| Order No. | Pack Qty. | Pack. | Price | Quantity | ||
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| 1314.1 | 8 unit(s) | cardboard | €243.90 |
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| 1314.2 | 5 unit(s) | cardboard | €635.00 |
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Downloads
Certificates of Analysis
Type analysis
| Particle size | 50-150 µm |
| Beads (cross-linked agarose) | 6 % |
| Ligand | IDA |
| Metal ion | nickel |
| Binding capacity (DHAK-6xHis) | 117 mg/ml |
| Activating agent | Epichlorohydrin |
| Stabilizer | 20 % ethanol |