Technical Data Sheet
Bovine Serum Albumin (BSA) Fraction V, NZ-Origin, 50 g
Storage temp. +4 °C
WGK 1
CAS No. 9048-46-8
EG-Nr. 232-936-2
Recommended if BSA with origin Australia or New Zealand is required.
Recommended as blocking and stabilising reagent in all antibody-mediated detection systems.
€188.00/Pack Qty.
excl. VAT. | 50 g per Pack Qty.
Art. No. 3737.2
Product details
For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system.
Molar extinction coefficient: 4,4x104 L x mol-1 x cm-1 (acc. to 0,667 L x mol-1 x cm-1 for a 0,1 % solution).
Bovine Serum Albumin (BSA) Fraction V, NZ-Origin >98 %, protease-free, IgG-free, nuclease-free
Albumin, highly purified and tested for the absence of immunoglobulins (IgGs), proteases, RNAses, and DNAses. Albumin, IgG-free is particularly recommended for use as stabilising reagent in antibody solutions, and as blocking reagent in all assays using detection systems via antibodies.
Also very well suited for stabilising of hot-start-PCRs mediated by antibodies, or as blocking reagent in antibody-detected hybridisations.
Tested for the presence of proteases, IgGs, DNases and RNases.
| Instructions for use, addition | for biochemistry and molecular biology |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 3737.1 | 10 g | plastic |
€68.50 |
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| 3737.2 | 50 g | plastic |
€188.00 |
|
|
| 3737.3 | 200 g | plastic |
€565.00 |
|
|
| 3737.4 | 500 g | plastic |
€1,125.00 |
|
|
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- Subtotal: 0.00
Downloads / MSDS
General information
Molar extinction coefficient: 4,4x104 L x mol-1 x cm-1 (acc. to 0,667 L x mol-1 x cm-1 for a 0,1 % solution).
BSA is a soluble monomeric protein with a molecular weight of 66.5 kDa. It is synthesized in the liver and extracted from the blood of cattle. In the bloodstream it serves as a binding and transport protein. BSA is used as a standard in protein quantification, as a blocking reagent, as a satabilizer in molecular applications and as a nutrient additive in cell cultures.
Purification process
The purification of the BSA is carried out using a far-reaching heat shock/diafiltration method that produces higher purity albumins than the usual Cohn crystallization. The process takes place in a closed system.
Albumin, NZ-Origin is particularly recommended when procedural regulations require the use of albumin of guaranteed origin Australia or New Zealand.
BSA, US-Origin is particularly recommended if the use of albumin with certified US-Origin is dictated by process regulations.
Albumin of European origin is produced from blood of European cattle herds. It is excellent for use in all assays and products manufactured for regions outside the USA, Australia and New Zealand.
Certificates of Analysis
Guarantee analysis
| Appearance | flakes |
| Colour | yellow to beige |
| Assay (HPLC) | >98 % |
| Dry extract | ≥94 % |
| Iron (Fe) | <15 mg/g |
| Sodium (Na) | <10 mg/g |
| Chloride (Cl) | <0.5 % |
| Solution (2 % in H2O) | soluble |
| pH value (2 % in H2O) | 6.5-7.5 |
| IgG (HPLC) | none detected |
| Protease | none detected |
| DNAse | none detected |
| RNAse | none detected |
| Origin | Australia/New Zealand |