Geneticin disulphate (G418 Sulphate), 10 g
Molar mass (M) 692,70 g/mol
Melting point (mp) 139 °C
Storage temp. +4 °C
CAS No. [108321-42-2]
Application: Selection of transfected eukaryotic cells. Resistance gene: Neomycin. Stock solution: 10-50 mg/ml in H2O or culture medium. Storage of stock solution: -20 °C. Working concentration: 50-1000 µg/ml, has to be established for each cell type.
excl. VAT. | 10 g per Pack Qty.
Art. No. 0239.4
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Geneticin disulphate (G418 Sulphate) ≥650 U/mg, for biochemistry
Inhibits protein biosynthesis by binding to ribosomes, particularly to eukaryotic 80S ribosomes. Through interference with the tRNA docking process, sequence errors appear and the elongating peptide chain is truncated. Geneticin disulphate unspecifically effects all cells, and is highly toxic for eukaryotic cells. Resistance is mediated by the bacterial enzyme aminoglycoside-3-phosphotransferase.
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