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ROTI®Prep Genomic DNA MINI, 10 preparations

for molecular biology
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Damage metals and burn body tissues; may cause serious eye damage. Safety: Avoid contact; wear safety spectacles and gloves. In the event of contact with eyes and skin, rinse with water.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
Examples of effect: Are harmful to aquatic organisms, toxic or highly toxic, acute or with long-term effects. Safety: To be disposed of as hazardous waste only, do not allow into the environment under any circumstances.
Danger
H225-H315-H317-H318-H334-H335-H336-H411
i highly flammable liquid and vapour, causes skin irritation, may cause an allergic skin reaction, causes serious eye damage, may cause allergy or asthma symptoms or breathing difficulties if inhaled, may cause respiratory irritation, may cause drowsiness or dizziness, toxic to aquatic life with long lasting effects
P210 P280 P303+P361+P353 P305+P351+P338 P342+P311
i keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking, wear protective gloves/protective clothing/eye protection/face protection, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/shower, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, if experiencing respiratory symptoms: Call a POISON CENTER/doctor
Pack Qty.
Pack.
Packaging
Boiling point (bp) 82 °C
Flash point (flp) <21 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
ADR 9 II
UN-Nr. 3316

Kit for isolation of genomic DNA from tissues, rodent tails, FFPE tissues, buccal swabs, cell cultures.
Preparation of up to 65 μg gDNA from up to 40 mg tissue per column.

For research use only. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro diagnostics.
Product details
Notes on the product
€38.90 /Pack Qty.

€30.30/Pack Qty.  Campaign price!

excl. VAT. | 1 kit per Pack Qty.

Art. No. 8472.1

In stock
Free of shipping costs from 125 €
24 hour direct shipment
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Personal technical advice on this product
Ms Hübner
Ms Hübner
+49 721 5606 - 1034

Product details


For research use only. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro diagnostics.



ROTI®Prep Genomic DNA MINI for molecular biology

Roti®Prep Genomic DNA MINI has been developed for the isolation of genomic DNA from various sources.
The isolation is carried out through the proven and reliable spin column technique, which is very easy to handle and needs only few steps.

  • Preparation by well-known mini spin-column system
  • Fast, easy and efficient
  • Preparation in approx. 15 minutes (excluding lysis)
  • Yield up to 65 μg gDNA, depending on source material

In addition to the especially on proteinase K adapted lysis buffer, the filtration spin column has been optimised, permitting particularly high extraction rates. Extraction from up to 40 mg tissue, therefore, allows a yield of up to μg µg DNA. The thereby extracted DNA is free of RNA and proteins, and can directly be stored or used for downstream applications.

Possible basic material:
  • Tissue (up to 40 mg)
  • Rodent tails (0,5-1 cm)
  • FFPE tissue (formalin-fixed, paraffin-embedded)
  • Buccal swabs
  • Cell culture (up to 5x106 cells)
8472_Banden
Figure:
Typical test gel following isolation of genomic DNA (A) and subsequent GAPDH-PCR (B) from rodent tail (frozen, 8 mm each).
Yield of gDNA (from left to right): 22,6 μg, 27,7 μg and 28 μg. Ratio A260:A280 = 1,97±0,01.

The kit contains

Lysis Buffer LSA, Binding Buffer BSN, Proteinase K, Washing Buffer WST, Elution Buffer EB, spin columns, collection tubes, elution tubes, detailed instructions-for-use.
Contents of this kit may not be bought separately.

Please order ethanol 99,8 % (p.a., Art. No. 9065), RNase A (Art. No. 7156, not for FFPE tissue), and for FFPE tissue xylole (Art. No. 4436) or octane separately.

8472_RotiPrepGenomic_Ablaufschema


ROTI®Prep Genomic DNA MINI
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Order No. Pack Qty. Pack. Packaging Price Quantity
8472.1 1 kit cardboard 10 préparations €30.30
8472.2 1 kit cardboard 50 preparations €112.90
8472.3 1 kit cardboard 250 preparations €443.80
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Notes on the product

For research use only. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro diagnostics.

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