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Ethidium bromide solution 0.5 % in dropper bottle, 15 ml, 1 x 15 ml

5 mg/ml in water, ready-to-use
Icon_ready-to-use Ready-to-use products and reagents
Pack Qty.
Pack.
Packaging
3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, Homidium bromide
Density (D) 1 g/cm³
Boiling point (bp) 100 °C
Melting point (mp) 0 °C
WGK 1

For staining of DNA/RNA after electrophoresis and for counterstaining of nuclei in histology and cytology.
Staining solution: add 5 drops of ethidium bromide solution to 500 ml buffer.
Store solution in the dark!

In spite of newly developed fluorescent staining reagents ethidium bromide remains the standard staining technic for nucleic acid in electrophoresis. Ethidium bromide is a carcinogenic substance which shouldn't come in contact with skin. In order to minimise this danger we offer ready-to-use ethidium bromide solutions in dropper bottles for easy handling and maximum safety.
Product details

€30.90/Pack Qty. 

excl. VAT. | 15 ml per Pack Qty.

Art. No. HP46.1

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Personal technical advice on this product
Ms Drotleff
Ms Drotleff
+49 721 5606 - 1037

Product details


In spite of newly developed fluorescent staining reagents ethidium bromide remains the standard staining technic for nucleic acid in electrophoresis. Ethidium bromide is a carcinogenic substance which shouldn't come in contact with skin. In order to minimise this danger we offer ready-to-use ethidium bromide solutions in dropper bottles for easy handling and maximum safety.


Ethidium bromide

Ethidium bromide is a strong mutagenic and toxic substance. For your own safety, always wear gloves and respiratory protection to prevent contamination when working with this substance. After use, dispose of solutions correctly. They may not be discarded into the environment.
The following procedure is recommended (acc. to INSERM, Dossier prevention, Number 2, 2nd Ed., September 88):
1. Collect and dispose of as hazardous waste.
2. Break down EtBr with potassium permanganate and HCI (P. Quillardet, M. Hofnung, Trends Genet. 4,89 [1988]).
3. Break down EtBr with hypophosphoric acid and sodium nitrite (Lunn and Sansone; Analyt. Biochem. 162, 453-458 [1987]).
4. Absorption of EtBr with Amberlite® XAD-16 (Lunn and Sansone) or with activated carbon (Bensaude, O. Method, Trends Genet. 4, 89-90 [1988]) from the solution.

We recomment disposure by SEKUROKA®Decon-Bags (Art. No. T856)



Ethidium bromide solution 0.5 % in dropper bottle 5 mg/ml in water, ready-to-use
Directions for use

In order to prepare a staining solution add 5 drops of ethidium bromide solution to 500 ml water, buffer, or electrophoresis running buffer, respectively.
Nuclei / nucleic acids are stained red.


Wavelength

Excitation maxima (unbound): 210 nm, 285 nm, 470 nm
Excitation maxima (bound to DNA): 330 nm, 500 nm. Excitation possible via DNA at 254 nm
Emission maximum: 605 nm.



Technical Information
Max. excitation (DNA-bound) 330 nm
500 nm
Max. excitation (unbound) 210 nm 
285 nm 
470 nm 
Emissions maximum 605 nm 
Use Addition to running buffer 
Ethidium bromide solution 0.5 % in dropper bottle
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Order No. Pack Qty. Pack. Packaging Price Quantity
HP46.1 15 ml plastic 1 x 15 ml €30.90
HP46.2 75 ml plastic 5 x 15 ml €107.50
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