Boiling point (bp) 100 °C
Flash point (flp) 34 °C
Storage temp. +4 °C
ADR 8 II
Application: Customary concentration in medium: 4-10 %
100 ml Kovacs' Reagent is sufficient for approx. 2 l medium.
excl. VAT. | 100 ml per Pack Qty.
Art. No. 2950.1
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Kovacs' Reagent for microbiology
Tryptophanase-positive microorganisms (e.g. E. coli, S. faecalis, Proteus sp.) that cleave tryptophane hereby release indole, which may be detected by reaction with dimethylamino benzaldehyde by forming a dark red dye.
Microorganisms must be incubated in a glucose-free broth with high concentrations of tryptophane present. Kovacs‘ Reagent is added to 24-48 h old cultures in a concentration of 0.2 to 0.5 ml per 5 ml broth. A colour shift to dark red shows presence of indole, thus confirming the inoculating colony as being indole-/tryptophanase-positive.
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|Appearance||yellowish, clear liquid|
|Hydrochloric acid (37 %)||25 %|