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Kovacs' Reagent

for microbiology
Examples of effect: Flammable; liquids form compounds with the air which can become explosive; produce flammable gases with water or can self-combust. Safety: Keep away from open flames and sources of heat; close containers tightly; store in a fire-safe manner.
Examples of effect: Damage metals and burn body tissues; may cause serious eye damage. Safety: Avoid contact; wear safety spectacles and gloves. In the event of contact with eyes and skin, rinse with water.
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Danger
H226-H290-H302-H315-H318-H335-H336
i flammable liquid and vapour, may be corrosive to metals, harmful if swallowed, causes skin irritation, causes serious eye damage, may cause respiratory irritation, may cause drowsiness or dizziness
P210 P280 P302+P352 P305+P351+P338
i keep away from heat. No smoking, wear protective gloves/eye protection, IF ON SKIN: Wash with plenty of soap and water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Pack Qty.
Pack.
Indole Reagent
Density (D) 0,92 g/cm³
Boiling point (bp) 100 °C
Flash point (flp) 34 °C
Storage temp. +4 °C
ADR 8 II
WGK 3
UN-Nr. 2920

Media supplement for detection of microbial indole.
Application: Customary concentration in medium: 4-10 %

100 ml Kovacs' Reagent is sufficient for approx. 2 l medium.

Tryptophanase-positive microorganisms (e.g. E. coli, S. faecalis, Proteus sp.) that cleave tryptophane hereby release indole, which may be detected by reaction with dimethylamino benzaldehyde by forming a dark red dye.
Product details
Type analysis

€44.90/Pack Qty. 

excl. VAT. | 100 ml per Pack Qty.

Art. No. 2950.1

In stock
Free of shipping costs from 125 €
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from 6 Pack Qty. €42.66/Pack Qty.
from 24 Pack Qty. €40.41/Pack Qty.
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Personal technical advice on this product
Ms Hübner
Ms Hübner
+49 721 5606 - 1034

Product details



Kovacs' Reagent for microbiology

Tryptophanase-positive microorganisms (e.g. E. coli, S. faecalis, Proteus sp.) that cleave tryptophane hereby release indole, which may be detected by reaction with dimethylamino benzaldehyde by forming a dark red dye.


Microorganisms must be incubated in a glucose-free broth with high concentrations of tryptophane present. Kovacs‘ Reagent is added to 24-48 h old cultures in a concentration of 0.2 to 0.5 ml per 5 ml broth. A colour shift to dark red shows presence of indole, thus confirming the inoculating colony as being indole-/tryptophanase-positive.



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Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Appearanceyellowish, clear liquid  
4-(Dimethylamino)benzaldehyde50 g/l  
1-Butanol75 %  
Hydrochloric acid (37 %)25 %  

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