anti-6-His-tag mouse monoclonal, 6His, 100 µg | Primary Antibodies | Antibodies | Western & ELISA | Protein Biology | Life Science

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Technical Data Sheet

Downloads / MSDS

Certificates of Analysis

anti-6-His-tag mouse monoclonal, 6His, 100 µg

From PROGEN

lyophilized, monoclonal, affinity purified

Downloads / MSDS

Certificates of Analysis

Bitte beachten: Abweichende Konditionen / Please note: Different conditions

Fits to / Accessories

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Product details

Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
High affinity to the respective protein tag
Robust & highly specific detection of the fusion protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

anti-6-His-tag mouse monoclonal, 6His lyophilized, monoclonal, affinity purified

The monoclonal 6-His antibody recognizes polyhistidine (6-His). The 6-His tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.

Application

Immunocytochemistry (ICC): assay dependent
Immunoprecipitation (IP): assay dependent
Western Blot (WB): 1:2,000-1:5,000 (0.125-0.05 μg/ml)

Western blot analysis of E. coli lysate containing 6-His-tagged protein with anti-6-His-tag antibody. Western blot analysis was performed on 160 ng, 80 ng or 20 ng of E. coli lysate containing 6-His-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-6-His-tag mouse monoclonal, 6His (Cat. No. 910HISL) was diluted in blocking buffer (antibody concentration 0.125 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

Western blot analysis of E. coli lysate containing 6-His-tagged protein with anti-6-His-tag antibody. Western blot analysis was performed on 50 ng or 25 ng of E. coli lysate containing 6-His-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-6-His-tag mouse monoclonal, 6His (Cat. No. 910HISL) was diluted in blocking buffer (antibody concentration 0.05 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

Not a medical device / Not an IVD product

Technical Information

Application	ICC/IF IP WB
Host species	mouse
Clonality	monoclonal
Purification	Affinity chromatography
Conjugate	uncojugated
Isotype	IgG1
Clone	6His
Target	6-His-Tag

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	Art. No.	Pack Qty.	Pack.	Price
	1NNA.1	25 µg	plastic	€112.50 €112.50
	1NNA.2	100 µg	plastic	€409.00 €409.00
In stock Available (note delivery time) In procurement No longer available Delivery date currently unknown