Technical Data Sheet
anti-DNA mouse monoclonal, AC-30-10, 1 ml
Boiling point (bp) ~100 °C
Storage temp. +4 °C
Transport temp. ambient temp.
€299.00/Pack Qty.
excl. VAT. | 1 ml per Pack Qty.
Art. No. 1NN5.2
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Product details
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
anti-DNA mouse monoclonal, AC-30-10 100 μg/ml, monoclonal, chromatographically purified
AC-30-10 stains DNA present in the nuclei and the mitochondria of cells and tissues. It is also a reliable positive control in assays for detection of DNA antibodies, especially for the detection of autoantibodies in autoimmune diseases. Due to the high sensitivity it can also be used for the detection of mycoplasma contamination in cell cultures.
Binding characteristics: reacts with all forms of native and denatured DNA; reactive also with lambda DNA and synthetic DNA.
Tested cultured cell lines: XLKE-A6 (Xenopus), RVF-SMC, PTK-2, HeLa.
Dot Blot: Assay dependent (on nitrocellulose membrane, after baking at 70°C)
Immunocytochemistry (ICC): 1:200 (0.5 μg/ml)
Immunohistochemistry (IHC) - frozen: 1:10 (10 μg/ml)
Immunohistochemistry (IHC) - paraffin: 1:10 (10 μg/ml, microwave treatment recommended)
| Application |
ICC/IF IHC Dot Blot |
| Host species | mouse |
| Clonality | monoclonal |
| Reactivity antibody | species-independent |
| Purification | Size-exclusion chromatography |
| Conjugate | uncojugated |
| Concentration | 100 µg/ml |
| Isotype | IgM |
| Clone | AC-30-10 |
| Target | double- and single-stranded DNA |
- Subtotal: 0.00
| Art. No. | Pack Qty. | Pack. | Price | Quantity | |
|---|---|---|---|---|---|
| 1NN5.1 | 0.2 ml | plastic |
€119.00 |
|
|
| 1NN5.2 | 1 ml | plastic |
€299.00 |
|
|
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