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ROTI®Free Stripping Buffer 2.0, 1 l

ready-to-use
Icon_ready-to-use Ready-to-use products and reagents
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H319
i causes serious eye irritation
P280 P305+P351+P338 P337+P313
i wear protective gloves/protective clothing/eye protection/face protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, if eye irritation persists: Get medical advice/attention
Pack Qty.
Pack.
Stripping Buffer
Density (D) 1,003 g/cm³
Boiling point (bp) 100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
WGK 1

Premixed buffer for gentle stripping of Western-Blot membranes after Chemoluminescence detection.
Suitable for nitrocellulose and PVDF-membranes.

Stripping buffer for efficient removal of primary and secondary antibodies, as well as chemoluminescence staining, from Western blot membranes. Due to the low pH of the buffer, the antibody conformation is altered, therefore gently removing all molecules bound to the epitopes (Legocki et. al. (1981) Analyt. Biochem. 111:385-92). Robust formulation without β-mercaptoethanol or DTT for use at room temperature. Suitable for nitrocellulose and PVDF membranes.
Product details
Notes on the product

€109.50/Pack Qty. 

excl. VAT. | 1 l per Pack Qty.

Art. No. 3319.2

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Free of shipping costs from 125 €
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from 6 Pack Qty. €104.02/Pack Qty.
from 24 Pack Qty. €98.55/Pack Qty.
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Personal technical advice on this product
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Product details



ROTI®Free Stripping Buffer 2.0 ready-to-use

Stripping buffer for efficient removal of primary and secondary antibodies, as well as chemoluminescence staining, from Western blot membranes. Due to the low pH of the buffer, the antibody conformation is altered, therefore gently removing all molecules bound to the epitopes (Legocki et. al. (1981) Analyt. Biochem. 111:385-92). Robust formulation without β-mercaptoethanol or DTT for use at room temperature. Suitable for nitrocellulose and PVDF membranes.

  • Effective
  • Gentle
  • Odour-free
  • Simple application w/o heating
  • Cost-efficient

Directions for use

Following rinsing steps in PBST- or TBST buffer, the membrane is incubated in ROTI®Free Stripping-Puffer 2.0 for 15-60 mins. at room temperature. After washing it again, the membrane may be blocked and probed anew. The particular time needed for complete stripping depends on the respective antibody/epitope pairing.



ROTI®Free Stripping Buffer 2.0
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3319.1 250 ml glass €41.90
3319.2 1 l glass €109.50
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Notes on the product

Remark: Contains SDS. Can be redissolved by heating (max. 45 °C).

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