ROTI®Aqua-P/C/I, 250 ml
Please consider that this product might be liable to some legal sanctions as for example to obtain an end user declaration or the prohibition of some applications. For details please do contact your counterpart in our sales department!
Ready-to-use products and reagents
Storage temp. +4 °C
ADR 6.1 II
WGK 3
UN-Nr. 2821
Store in the dark!
The low pH results in interphase enrichment of the DNA, reducing DNA contamination in the RNA eluate.
€48.90/Pack Qty.
excl. VAT. | 250 ml per Pack Qty.
Art. No. X985.1
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Product details
- Reduce exposure to toxic chemicals
- Prepared from phenol of highest purity
- Packed under argon for maximum stability
- Successfully tried and tested in many research laboratories
Please note our Technical Information Brochure on the Internet next to the products:
Phenolic DNA Isolation
Background and protocol
Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses.
For distinct phase separation, ROTI®Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl.
Draw solution from lower phase. Do not shake before use!
ROTI®Aqua-P/C/I ready-to-use, for RNA extraction
ROTI®Aqua-P/C/I is the optimal choice for RNA isolation and other procedures where a low phenol pH value is desired.
The low pH results in interphase enrichment of the DNA, reducing DNA contamination in the RNA eluate.
Addition of 1 volume ROTI®Aqua-P/C/I and thorough mixing. Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. The acid pH-value causes a depletion of the DNA, so that only little contaminating DNA is isolated. Following centrifugation, the RNA containing solution may be retrieved as upper phase.
For distinct phase separation, ROTI®Aqua-P/C/I needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 12 drops of 1 N HCl.
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| Order No. | Pack Qty. | Pack. | Price | Quantity | ||
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| X985.1 | 250 ml | glass | €48.90 |
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| X985.2 | 500 ml | glass | €74.90 |
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| X985.3 | 100 ml | glass | €27.90 |
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